We identified, by two-dimensional electrophoretic analysis and microsequencing, a protein of Chlamydia trachomatis elementary bodies which corresponds to the polypeptide (pgp3) encoded by open reading frame 3 (ORF3). Amino acid analysis showed that the first residue (dry) found in the native protein is the one encoded by the second ORF3 codon, implying a typical bacterial removal of the first Met residue. Relatively large amounts of recombinant pgp3 (r-pgp3) in a stable, water-soluble form were obtained by overexpressing ORF3 in Escherichia call and purifying the product from periplasmic extracts under nondenaturing conditions. These r-pgp3 preparations allowed specific detection of anti-pgp3 antibodies by enzyme-linked immunosorbent assay. Analysis of a group of 170 sera from healthy blood donors and from patients who were seropositive or -negative for C. trachomatis and Chlamydia pneumoniae showed that an immune response to pgp3 occurs in the majority (ca. 81%) of patients with sexually transmitted diseases who are seropositive for C, trachomatis and generally correlates with the response to tell surface antigens. No reaction between r-pgp3 and C. pneumoniae-positive sera was detected.

Comanducci, M., Manetti, R., Bini, L., Santucci, A., V., P., Cevenini, R., et al. (1994). Humoral immune response to plasmid protein pgp3 in patients with Chlamydia trachomatis infection. INFECTION AND IMMUNITY, 62(12), 5491-5497 [10.1128/IAI.62.12.5491-5497.1994].

Humoral immune response to plasmid protein pgp3 in patients with Chlamydia trachomatis infection.

BINI, LUCA;SANTUCCI, ANNALISA;
1994

Abstract

We identified, by two-dimensional electrophoretic analysis and microsequencing, a protein of Chlamydia trachomatis elementary bodies which corresponds to the polypeptide (pgp3) encoded by open reading frame 3 (ORF3). Amino acid analysis showed that the first residue (dry) found in the native protein is the one encoded by the second ORF3 codon, implying a typical bacterial removal of the first Met residue. Relatively large amounts of recombinant pgp3 (r-pgp3) in a stable, water-soluble form were obtained by overexpressing ORF3 in Escherichia call and purifying the product from periplasmic extracts under nondenaturing conditions. These r-pgp3 preparations allowed specific detection of anti-pgp3 antibodies by enzyme-linked immunosorbent assay. Analysis of a group of 170 sera from healthy blood donors and from patients who were seropositive or -negative for C. trachomatis and Chlamydia pneumoniae showed that an immune response to pgp3 occurs in the majority (ca. 81%) of patients with sexually transmitted diseases who are seropositive for C, trachomatis and generally correlates with the response to tell surface antigens. No reaction between r-pgp3 and C. pneumoniae-positive sera was detected.
Comanducci, M., Manetti, R., Bini, L., Santucci, A., V., P., Cevenini, R., et al. (1994). Humoral immune response to plasmid protein pgp3 in patients with Chlamydia trachomatis infection. INFECTION AND IMMUNITY, 62(12), 5491-5497 [10.1128/IAI.62.12.5491-5497.1994].
File in questo prodotto:
File Dimensione Formato  
iai.62.12.5491-5497.1994.pdf

non disponibili

Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 1.66 MB
Formato Adobe PDF
1.66 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/3634