Mutated cellular ras genes have been detected in many tumor types, though with varying frequencies: for example, it is well known that between 40-50 % of colon carcinoma show mutations in one allele of the Kirsten ras gene, involving preferentially the codon 12 in which the most frequent change is the transition GGT to GAT; they may be found in the stools of the colorectal cancer patients. Purpose of our study is that to analyze stools of the normal subjects and of patients with colorectal tumor for the transition GGT to GAT in codon 12 of K-ras and, in the present paper, we report the most reproducible procedure to obtain purified DNA from stools.
Pagani, R., Porcelli, B., Frosi, B., Civitelli, S., Mancini, A., Civitelli, B., et al. (1996). Analysis of K-ras mutations in the stool of patients with colorectal cancer. MINERVA BIOTECNOLOGICA, 8(3), 142-144.
Analysis of K-ras mutations in the stool of patients with colorectal cancer
Pagani R.;Porcelli B.;Civitelli S.;
1996-01-01
Abstract
Mutated cellular ras genes have been detected in many tumor types, though with varying frequencies: for example, it is well known that between 40-50 % of colon carcinoma show mutations in one allele of the Kirsten ras gene, involving preferentially the codon 12 in which the most frequent change is the transition GGT to GAT; they may be found in the stools of the colorectal cancer patients. Purpose of our study is that to analyze stools of the normal subjects and of patients with colorectal tumor for the transition GGT to GAT in codon 12 of K-ras and, in the present paper, we report the most reproducible procedure to obtain purified DNA from stools.
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https://hdl.handle.net/11365/35291
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