Five different V3 domains of HIV-1 gp120 were expressed on the surface of the gram-positive bacterium Streptococcus gordonii, a model live vector for vaccine delivery. Sera of HIV-1-infected individuals and human monoclonal antibodies specifically recognized the gp120 sequences on the bacterial surface. Recombinant V3 from the reference HIV-1 strain MN was also shown to retain a conformation that allowed reaction with a conformation-specific monoclonal antibody. A V3-specific serum antibody response was detected in mice immunized both by subcutaneous injection and by vaginal colonization. V3-specific IgG2a antibodies, suggestive of a Th1 response, were found in the sera of mice colonized by recombinant bacteria.

Oggioni, M.r., Medaglini, D., Romano, L., Peruzzi, F., Maggi, T., Lozzi, L., et al. (1999). Antigenicity and immunogenicity of the V3 domain of HIV-1 gp120 expressed on the surface of Streptococcus gordonii. AIDS Res Hum Retroviruses. AIDS RESEARCH AND HUMAN RETROVIRUSES, 15, 451-459 [10.1089/088922299311204].

Antigenicity and immunogenicity of the V3 domain of HIV-1 gp120 expressed on the surface of Streptococcus gordonii. AIDS Res Hum Retroviruses

MEDAGLINI, DONATA;ROMANO, LAURA;LOZZI, LUISA;BRACCI, LUISA;ZAZZI, MAURIZIO;VALENSIN, PIER EGISTO;POZZI, GIANNI
1999-01-01

Abstract

Five different V3 domains of HIV-1 gp120 were expressed on the surface of the gram-positive bacterium Streptococcus gordonii, a model live vector for vaccine delivery. Sera of HIV-1-infected individuals and human monoclonal antibodies specifically recognized the gp120 sequences on the bacterial surface. Recombinant V3 from the reference HIV-1 strain MN was also shown to retain a conformation that allowed reaction with a conformation-specific monoclonal antibody. A V3-specific serum antibody response was detected in mice immunized both by subcutaneous injection and by vaginal colonization. V3-specific IgG2a antibodies, suggestive of a Th1 response, were found in the sera of mice colonized by recombinant bacteria.
Oggioni, M.r., Medaglini, D., Romano, L., Peruzzi, F., Maggi, T., Lozzi, L., et al. (1999). Antigenicity and immunogenicity of the V3 domain of HIV-1 gp120 expressed on the surface of Streptococcus gordonii. AIDS Res Hum Retroviruses. AIDS RESEARCH AND HUMAN RETROVIRUSES, 15, 451-459 [10.1089/088922299311204].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/35130
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