We present the results of a study of the high-performance liquid chromatography of two vitamin B6 derivatives: the carbamoyl derivatives of pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate. These compounds, obtained by condensation of either pyridoxal 5'-phosphate or pyridoxamine 5'-phosphate with either carbamoyl phosphate or potassium cyanate, were separated on an octadecyl silica column with a mobile phase consisting of 0.01 M potassium phosphate (pH 5.0)-methanol (98:2); detection was at 254 nm. The method was sensitive, fast and precise.
Terzuoli, L., Pagani, R., Leoncini, R., Vannoni, D., Marinello, E. (1991). High-performance liquid chromatography of two derivatives of vitamin B6, the carbamoyl derivatives of pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate. JOURNAL OF CHROMATOGRAPHY, 547(1-2), 472-477 [10.1016/S0021-9673(01)88675-1].
High-performance liquid chromatography of two derivatives of vitamin B6, the carbamoyl derivatives of pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate
Terzuoli L.;Pagani R.;Leoncini R.;Vannoni D.;Marinello E.
1991-01-01
Abstract
We present the results of a study of the high-performance liquid chromatography of two vitamin B6 derivatives: the carbamoyl derivatives of pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate. These compounds, obtained by condensation of either pyridoxal 5'-phosphate or pyridoxamine 5'-phosphate with either carbamoyl phosphate or potassium cyanate, were separated on an octadecyl silica column with a mobile phase consisting of 0.01 M potassium phosphate (pH 5.0)-methanol (98:2); detection was at 254 nm. The method was sensitive, fast and precise.
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https://hdl.handle.net/11365/35092
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