The paramagnetic contributions to the spin-lattice relaxation rates of biotin C-13 nuclei, induced by the presence in the water/DMSO solution of the TEMPOL nitroxide, have been analysed in the interaction with avidin. The paramagnetic relaxation data, obtained at different temperatures, indicate that the average solvent/spin-label exposure of biotin carbons is consistent with the conformational features previously observed for the complex in the crystal. The analysis of the paramagnetic perturbation profiles, derived from C-13 spin lattice relaxation measurements, seems to be highly informative of the sterical aspects of interaction processes of large biopolymers with their ligands.
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|Titolo:||NMR structural investigation of the biotin-avidin complex: A C-13 NMR paramagnetic relaxation study|
|Citazione:||M., S., A., V., A., B., M., R., & Niccolai, N. (1997). NMR structural investigation of the biotin-avidin complex: A C-13 NMR paramagnetic relaxation study. SPECTROSCOPY LETTERS, 30, 1201-1209.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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