The water proton Nuclear Magnetic Resonance (NMR) spin-lattice relaxation rates were measured in suspension of uninfected and Herpes simplex virus type 2-infected WI-38 diploid cells and shown to allow reliable detection of (i) the early signs of productive infection, and (ii) the tendency to undergo either spontaneous or virus-induced transformation. In addition, NMR investigation of several transformed cell lines currently and previously established from WI-38 cells clearly indicated that the NMR relaxation parameter is a suitable tool for assessment of the level of malignancy, as revealed by in vivo oncogenicity tests in immunosuppressed mice.

Valensin, P.E., BIANCHI BANDINELLI, M.L., Zazzi, M., Gaggelli, E., & Valensin, G. (1990). NMR investigation of cell cultures: early detection of infection by Herpes simplex virus type 2 and transformation. MICROBIOLOGICA, 13, 11-19.

NMR investigation of cell cultures: early detection of infection by Herpes simplex virus type 2 and transformation

VALENSIN, PIER EGISTO;BIANCHI BANDINELLI, MARIA LETIZIA;ZAZZI, MAURIZIO;GAGGELLI, ELENA;VALENSIN, GIANNI
1990

Abstract

The water proton Nuclear Magnetic Resonance (NMR) spin-lattice relaxation rates were measured in suspension of uninfected and Herpes simplex virus type 2-infected WI-38 diploid cells and shown to allow reliable detection of (i) the early signs of productive infection, and (ii) the tendency to undergo either spontaneous or virus-induced transformation. In addition, NMR investigation of several transformed cell lines currently and previously established from WI-38 cells clearly indicated that the NMR relaxation parameter is a suitable tool for assessment of the level of malignancy, as revealed by in vivo oncogenicity tests in immunosuppressed mice.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/34718
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