The water proton Nuclear Magnetic Resonance (NMR) spin-lattice relaxation rates were measured in suspension of uninfected and Herpes simplex virus type 2-infected WI-38 diploid cells and shown to allow reliable detection of (i) the early signs of productive infection, and (ii) the tendency to undergo either spontaneous or virus-induced transformation. In addition, NMR investigation of several transformed cell lines currently and previously established from WI-38 cells clearly indicated that the NMR relaxation parameter is a suitable tool for assessment of the level of malignancy, as revealed by in vivo oncogenicity tests in immunosuppressed mice.
Valensin, P.E., BIANCHI BANDINELLI, M.L., Zazzi, M., Gaggelli, E., Valensin, G. (1990). NMR investigation of cell cultures: early detection of infection by Herpes simplex virus type 2 and transformation. MICROBIOLOGICA, 13(1), 11-19.
NMR investigation of cell cultures: early detection of infection by Herpes simplex virus type 2 and transformation
VALENSIN, P. E.;BIANCHI BANDINELLI, M. L.;ZAZZI, M.;GAGGELLI, E.;VALENSIN, G.
1990-01-01
Abstract
The water proton Nuclear Magnetic Resonance (NMR) spin-lattice relaxation rates were measured in suspension of uninfected and Herpes simplex virus type 2-infected WI-38 diploid cells and shown to allow reliable detection of (i) the early signs of productive infection, and (ii) the tendency to undergo either spontaneous or virus-induced transformation. In addition, NMR investigation of several transformed cell lines currently and previously established from WI-38 cells clearly indicated that the NMR relaxation parameter is a suitable tool for assessment of the level of malignancy, as revealed by in vivo oncogenicity tests in immunosuppressed mice.
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https://hdl.handle.net/11365/34718
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