Phage display technology represents a powerful tool for the identification of peptides reacting with disease-related antibodies present in human sera. The application of this technology to type 1 diabetes could provide a set of novel reagents for diabetes prediction and could also lead to the identification of novel autoantigens or even of environmental factors possibly causing the disease. In the present study, sera of prediabetic and high risk individuals were used to select candidate peptides from phage-displayed random peptide libraries. Diabetes specific phage clones were then identified from these through screening and counter screening, using sera from diabetic and non-diabetic individuals. The results presented in this paper demonstrate the feasibility of this methodology to identify peptides reacting preferentially with antibodies present in the serum of diabetic patients.

Mennuni, C., Santini, C., Dotta, F., Farilla, L., Di Mario, U., Fierabracci, A., et al. (1996). Selection of phage-displayed peptides mimicking type 1 diabetes-specific epitopes. JOURNAL OF AUTOIMMUNITY, 9(3), 431-436 [10.1006/jaut.1996.0060].

Selection of phage-displayed peptides mimicking type 1 diabetes-specific epitopes

DOTTA, FRANCESCO;
1996-01-01

Abstract

Phage display technology represents a powerful tool for the identification of peptides reacting with disease-related antibodies present in human sera. The application of this technology to type 1 diabetes could provide a set of novel reagents for diabetes prediction and could also lead to the identification of novel autoantigens or even of environmental factors possibly causing the disease. In the present study, sera of prediabetic and high risk individuals were used to select candidate peptides from phage-displayed random peptide libraries. Diabetes specific phage clones were then identified from these through screening and counter screening, using sera from diabetic and non-diabetic individuals. The results presented in this paper demonstrate the feasibility of this methodology to identify peptides reacting preferentially with antibodies present in the serum of diabetic patients.
1996
Mennuni, C., Santini, C., Dotta, F., Farilla, L., Di Mario, U., Fierabracci, A., et al. (1996). Selection of phage-displayed peptides mimicking type 1 diabetes-specific epitopes. JOURNAL OF AUTOIMMUNITY, 9(3), 431-436 [10.1006/jaut.1996.0060].
File in questo prodotto:
File Dimensione Formato  
Mennuni et al J Autoimmunity 1996.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 370.56 kB
Formato Adobe PDF
370.56 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/33830
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo