Generation of primed T cells is crucial for the development of optimal vaccination strategies. Using a TCR-transgenic CD4(+) and CD8(+) T cell adoptive transfer model, we demonstrate that a single nasal immunization with recombinant Streptococcus gordonii induces antigen-specific primed T cells in lymph nodes draining the genital and intestinal tracts with about 80% of CD4(+) and 50% of CD8(+) proliferating cells. T cell clonal expansion was also observed in cervical lymph nodes, draining the immunization site, and in the spleen. The modulation of CD44 and CD45RB marker expression indicated that proliferating T cells were activated. Proliferation in distal mesenteric and iliac lymph nodes and in the spleen was observed 5 days after nasal immunization, while in draining cervical lymph nodes proliferation peaked already at day 3. The division profile of transgenic T cells observed in iliac and mesenteric lymph nodes was discontinuous, showing the lack of early cell divisions. The kinetics of T cell clonal expansion, the discontinuous division profile and the modulation of migration markers such as CD62L suggest that activated antigen-specific T cells disseminate from the immunization site to distal intestinal and genital tracts. These data demonstrate the efficacy of nasal immunization with recombinant S. gordonii in eliciting CD4(+) and CD8(+) T cell priming not only in draining sites, but also in the genital and intestinal tracts and in the spleen.

Ciabattini, A., Pettini, E., Arsenijevic, S., Pozzi, G., Medaglini, D. (2010). Intranasal immunization with vaccine vector Streptococcus gordonii elicits primed CD4(+) and CD8(+) T cells in the genital and intestinal tracts. VACCINE, 28(5), 1226-1233 [10.1016/j.vaccine.2009.11.021].

Intranasal immunization with vaccine vector Streptococcus gordonii elicits primed CD4(+) and CD8(+) T cells in the genital and intestinal tracts.

CIABATTINI, ANNALISA;PETTINI, ELENA;POZZI, GIANNI;MEDAGLINI, DONATA
2010-01-01

Abstract

Generation of primed T cells is crucial for the development of optimal vaccination strategies. Using a TCR-transgenic CD4(+) and CD8(+) T cell adoptive transfer model, we demonstrate that a single nasal immunization with recombinant Streptococcus gordonii induces antigen-specific primed T cells in lymph nodes draining the genital and intestinal tracts with about 80% of CD4(+) and 50% of CD8(+) proliferating cells. T cell clonal expansion was also observed in cervical lymph nodes, draining the immunization site, and in the spleen. The modulation of CD44 and CD45RB marker expression indicated that proliferating T cells were activated. Proliferation in distal mesenteric and iliac lymph nodes and in the spleen was observed 5 days after nasal immunization, while in draining cervical lymph nodes proliferation peaked already at day 3. The division profile of transgenic T cells observed in iliac and mesenteric lymph nodes was discontinuous, showing the lack of early cell divisions. The kinetics of T cell clonal expansion, the discontinuous division profile and the modulation of migration markers such as CD62L suggest that activated antigen-specific T cells disseminate from the immunization site to distal intestinal and genital tracts. These data demonstrate the efficacy of nasal immunization with recombinant S. gordonii in eliciting CD4(+) and CD8(+) T cell priming not only in draining sites, but also in the genital and intestinal tracts and in the spleen.
Ciabattini, A., Pettini, E., Arsenijevic, S., Pozzi, G., Medaglini, D. (2010). Intranasal immunization with vaccine vector Streptococcus gordonii elicits primed CD4(+) and CD8(+) T cells in the genital and intestinal tracts. VACCINE, 28(5), 1226-1233 [10.1016/j.vaccine.2009.11.021].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/32555
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