Direct sequencing of polymerase chain reaction (PCR)-generated templates is a commonly used technique in molecular biology laboratories. We describe an improved method for direct sequencing of PCR fragments longer than 20 kb obtained with a commercial mixture of Taq and Pwo DNA polymerases. The sequencing protocol was optimized for an automated infrared DNA sequencer, consistently yielding long reads (500-600 bases).
Iannelli, F., Giunti, L., Pozzi, G. (1998). Direct sequencing of long PCR fragments. MOLECULAR BIOTECHNOLOGY, 10(2), 183-185 [10.1007/BF02760864].
Direct sequencing of long PCR fragments
Iannelli, Francesco;Pozzi, Gianni
1998-01-01
Abstract
Direct sequencing of polymerase chain reaction (PCR)-generated templates is a commonly used technique in molecular biology laboratories. We describe an improved method for direct sequencing of PCR fragments longer than 20 kb obtained with a commercial mixture of Taq and Pwo DNA polymerases. The sequencing protocol was optimized for an automated infrared DNA sequencer, consistently yielding long reads (500-600 bases).
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https://hdl.handle.net/11365/32431
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