Recent studies reported the possibility of detecting prostate adenocarcinoma and malignant melanoma cells in peripheral blood using RT-PCR of prostatic specific antigen (PSA), prostatic specific membrane antigen (PSMA) and Tyrosinase mRNAs. The PCR results showed high variability, ranging between 0% and 100% of positivity in patients with advanced disease. Our purpose was to evaluate the presence of tumor marker mRNAs in peripheral blood of prostate cancer and melanoma patients by means of RT-nested-PCR. We tested 70 and 36 peripheral blood samples from prostate carcinoma and malignant melanoma patients, respectively. The RT-PCR analysis showed the presence of PSA cDNA in 9 out of 70 (12.9%); PSMA cDNA in 14 out of 70 (20%); and Tyrosinase cDNA in 2 out of 36 (5.5%) peripheral blood samples from melanoma patients. Our study confirms the applicability of this sensitive method to monitor disease status. Although, the RT-nested-PCR of Tyrosinase is able to detect neoplastic cells in peripheral blood specimens, we suggest the necessity of a great caution in interpreting PCR results when the nested method has been used.

Sardi, I., Moretti, S., Arrigucci, S., Guazzelli, R., Montali, E., Ponchietti, R. (1999). The role of the detection of hematogenous micrometastasis in prostate adenicarcinoma and malignant melanoma by RT – PCR. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 3(4), 417-418 [10.3892/ijmm.3.4.417].

The role of the detection of hematogenous micrometastasis in prostate adenicarcinoma and malignant melanoma by RT – PCR

PONCHIETTI, ROBERTO
1999-01-01

Abstract

Recent studies reported the possibility of detecting prostate adenocarcinoma and malignant melanoma cells in peripheral blood using RT-PCR of prostatic specific antigen (PSA), prostatic specific membrane antigen (PSMA) and Tyrosinase mRNAs. The PCR results showed high variability, ranging between 0% and 100% of positivity in patients with advanced disease. Our purpose was to evaluate the presence of tumor marker mRNAs in peripheral blood of prostate cancer and melanoma patients by means of RT-nested-PCR. We tested 70 and 36 peripheral blood samples from prostate carcinoma and malignant melanoma patients, respectively. The RT-PCR analysis showed the presence of PSA cDNA in 9 out of 70 (12.9%); PSMA cDNA in 14 out of 70 (20%); and Tyrosinase cDNA in 2 out of 36 (5.5%) peripheral blood samples from melanoma patients. Our study confirms the applicability of this sensitive method to monitor disease status. Although, the RT-nested-PCR of Tyrosinase is able to detect neoplastic cells in peripheral blood specimens, we suggest the necessity of a great caution in interpreting PCR results when the nested method has been used.
1999
Sardi, I., Moretti, S., Arrigucci, S., Guazzelli, R., Montali, E., Ponchietti, R. (1999). The role of the detection of hematogenous micrometastasis in prostate adenicarcinoma and malignant melanoma by RT – PCR. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 3(4), 417-418 [10.3892/ijmm.3.4.417].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/32102
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