To determine the sensitivity of a nested PCR procedure for detecting human immunodeficiency virus type 1 DNA in clinical specimens, 553 peripheral blood mononuclear cell samples obtained from 268 human immunodeficiency virus type 1-seropositive subjects were assayed by use of two independent primer sets for each sample. Overall, 1,088 of 1,106 (98.37%) reactions were positive. Investigation of the negative reactions showed that a low viral burden in some infected subjects, rather than primer-template mismatches, was the primary cause for the false-negative PCR results.
Zazzi, M., Romano, L., Catucci, M., DE MILITO, A., Almi, P., Gonnelli, A., et al. (1995). Low human immunodeficiency virus type 1 (HIV-1)DNA burden as a major cause for failure to detect HIV-1 DNA in clinical specimens by PCR. JOURNAL OF CLINICAL MICROBIOLOGY, 33(1), 205-208 [10.1128/jcm.33.1.205-208.1995].
Low human immunodeficiency virus type 1 (HIV-1)DNA burden as a major cause for failure to detect HIV-1 DNA in clinical specimens by PCR
ZAZZI M.;VALENSIN P. E.
1995-01-01
Abstract
To determine the sensitivity of a nested PCR procedure for detecting human immunodeficiency virus type 1 DNA in clinical specimens, 553 peripheral blood mononuclear cell samples obtained from 268 human immunodeficiency virus type 1-seropositive subjects were assayed by use of two independent primer sets for each sample. Overall, 1,088 of 1,106 (98.37%) reactions were positive. Investigation of the negative reactions showed that a low viral burden in some infected subjects, rather than primer-template mismatches, was the primary cause for the false-negative PCR results.File | Dimensione | Formato | |
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https://hdl.handle.net/11365/31908
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