Endothelial cells culture can be considered a reliable method for investigating about granulation tissue production in wound healing and for evaluating the pharmacological action of some chemicals on granulation tissue development. Endothelial cells have been obtained from human umbilical cords after trypsin treatment and their endothelial origin has been demonstrated by light microscopy, by immunofluorescence against factor VIII associated protein and by the platelet adhesion assay. The influence of fibronectin as substratum and of hyaluronic acid as soluble factor on adhesion and growth of endothelial cells has been investigated. Both these substances, but especially hyaluronate, determine a better attachment and an increase in the growth rate when compared with control cultures plated on plastic substratum and without hyaluronic acid in the culture medium.

Mancianti, M.L., Fimiani, M., Castelli, A., Raffaelli, M., Perotti, R., Valentino, A. (1982). Endothelial cell culture as a model for the study of wound healing [Colture di cellule endoteliali come modello per lo studio della cicatrizzazione]. BOLLETTINO DELLA SOCIETA' ITALIANA DI BIOLOGIA SPERIMENTALE, 60(3), 473-478.

Endothelial cell culture as a model for the study of wound healing [Colture di cellule endoteliali come modello per lo studio della cicatrizzazione]

Fimiani M.;
1982-01-01

Abstract

Endothelial cells culture can be considered a reliable method for investigating about granulation tissue production in wound healing and for evaluating the pharmacological action of some chemicals on granulation tissue development. Endothelial cells have been obtained from human umbilical cords after trypsin treatment and their endothelial origin has been demonstrated by light microscopy, by immunofluorescence against factor VIII associated protein and by the platelet adhesion assay. The influence of fibronectin as substratum and of hyaluronic acid as soluble factor on adhesion and growth of endothelial cells has been investigated. Both these substances, but especially hyaluronate, determine a better attachment and an increase in the growth rate when compared with control cultures plated on plastic substratum and without hyaluronic acid in the culture medium.
1982
Mancianti, M.L., Fimiani, M., Castelli, A., Raffaelli, M., Perotti, R., Valentino, A. (1982). Endothelial cell culture as a model for the study of wound healing [Colture di cellule endoteliali come modello per lo studio della cicatrizzazione]. BOLLETTINO DELLA SOCIETA' ITALIANA DI BIOLOGIA SPERIMENTALE, 60(3), 473-478.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/31371
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo