The addition of G-6-Pi to the incubation system for MgATP-dependent calcium transport in liver microsomes results in a marked stimulation of Ca2+ uptake. At physiological pH values (7.2-7.4), the G-6-Pi stimulated calcium uptake is maximal and equals that obtained with oxalate at pH 6.8. In the system for the G-6-Pi-stimulated calcium uptake, G-6-Pi is actively hydrolyzed by the glucose 6-phosphatase activity of liver microsomes. Such an activity is not influenced by the concomitant calcium uptake. After the incubation of the system for the MgATP-dependent microsomal calcium transport in the presence of G-6-Pi, Pi and calcium are found in equal concentrations, on a molar base, in the recovered microsomal fraction. These results are interpreted in the light of a possible cooperative activity between the energy-dependent calcium pump of liver microsomes and the glucose 6-phosphatase multicomponent system.

Benedetti, A., Fulceri, R., Comporti, M. (1984). Funzionamento accoppiato delle pompe del calcio e dell'attività glucoso-6 fosfatasica nei microsomi epatici: risultati preliminari [Functional coupling of the calcium pump and glucose 6-phosphatase activity in liver microsomes: preliminary results]. BOLLETTINO DELLA SOCIETA' ITALIANA DI BIOLOGIA SPERIMENTALE, 60(7), 1317-1323.

Funzionamento accoppiato delle pompe del calcio e dell'attività glucoso-6 fosfatasica nei microsomi epatici: risultati preliminari [Functional coupling of the calcium pump and glucose 6-phosphatase activity in liver microsomes: preliminary results]

Benedetti, A.;Fulceri, R.;Comporti, M.
1984-01-01

Abstract

The addition of G-6-Pi to the incubation system for MgATP-dependent calcium transport in liver microsomes results in a marked stimulation of Ca2+ uptake. At physiological pH values (7.2-7.4), the G-6-Pi stimulated calcium uptake is maximal and equals that obtained with oxalate at pH 6.8. In the system for the G-6-Pi-stimulated calcium uptake, G-6-Pi is actively hydrolyzed by the glucose 6-phosphatase activity of liver microsomes. Such an activity is not influenced by the concomitant calcium uptake. After the incubation of the system for the MgATP-dependent microsomal calcium transport in the presence of G-6-Pi, Pi and calcium are found in equal concentrations, on a molar base, in the recovered microsomal fraction. These results are interpreted in the light of a possible cooperative activity between the energy-dependent calcium pump of liver microsomes and the glucose 6-phosphatase multicomponent system.
1984
Benedetti, A., Fulceri, R., Comporti, M. (1984). Funzionamento accoppiato delle pompe del calcio e dell'attività glucoso-6 fosfatasica nei microsomi epatici: risultati preliminari [Functional coupling of the calcium pump and glucose 6-phosphatase activity in liver microsomes: preliminary results]. BOLLETTINO DELLA SOCIETA' ITALIANA DI BIOLOGIA SPERIMENTALE, 60(7), 1317-1323.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/31156
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