1. MgATP-dependent Ca2+ uptake by rat liver microsomal preparations and permeabilized hepatocytes was measured in the presence or absence of P(i). 2. Monitoring of free Ca2+ in incubation systems with a Ca2+ electrode in the presence of P(i) (2-7 nM) revealed a biphasic Ca2+ uptake, with the onset of a second, P(i)-dependent, Ca2+ accumulation. 3. Increasing P(i) concentrations (up to 10 mM) caused a progressive enlargement of 45Ca2+-loading capacity of microsomal fractions. 4. As a result of P(i) stimulation of active Ca2+ uptake, [32P]P(i) and 45Ca2+ were co-accumulated. 5. Experiments with permeabilized hepatocytes revealed that the amount of Ca2+ releasable by myo-inositol 1,4,5-trisphosphate is unaffected by P(i).

Fulceri, R., Bellomo, G., Gamberucci, A., Benedetti, A. (1990). MgATP-dependent accumulation of calcium ions and inorganic phosphate in a liver reticular pool. BIOCHEMICAL JOURNAL, 272(2), 549-552 [10.1042/bj2720549].

MgATP-dependent accumulation of calcium ions and inorganic phosphate in a liver reticular pool

Fulceri, R.;Gamberucci, A.;Benedetti, A.
1990-01-01

Abstract

1. MgATP-dependent Ca2+ uptake by rat liver microsomal preparations and permeabilized hepatocytes was measured in the presence or absence of P(i). 2. Monitoring of free Ca2+ in incubation systems with a Ca2+ electrode in the presence of P(i) (2-7 nM) revealed a biphasic Ca2+ uptake, with the onset of a second, P(i)-dependent, Ca2+ accumulation. 3. Increasing P(i) concentrations (up to 10 mM) caused a progressive enlargement of 45Ca2+-loading capacity of microsomal fractions. 4. As a result of P(i) stimulation of active Ca2+ uptake, [32P]P(i) and 45Ca2+ were co-accumulated. 5. Experiments with permeabilized hepatocytes revealed that the amount of Ca2+ releasable by myo-inositol 1,4,5-trisphosphate is unaffected by P(i).
1990
Fulceri, R., Bellomo, G., Gamberucci, A., Benedetti, A. (1990). MgATP-dependent accumulation of calcium ions and inorganic phosphate in a liver reticular pool. BIOCHEMICAL JOURNAL, 272(2), 549-552 [10.1042/bj2720549].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/31065
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