The interaction of apramycin with an RNA fragment mimicking the bacterial ribosomal A site was investigated by NMR spectroscopy, mainly by using the transferred NOE technique. The fact that only the RNA-bound antibiotic shows nonzero NOE effects, allowed us to gain structural details of bound apramycin despite the fast exchange conditions between the free and RNA-bound forms. The dissociation constant of the apramycin–RNA complex was evaluated by CD spectroscopy.
Balenci, D., D'Amelio, N., Gaggelli, E., Gaggelli, N., Cellai, L., Molteni, E., et al. (2010). Structural features of apramycin bound at the bacterial ribosome a site as detected by NMR and CD spectroscopy. CHEMBIOCHEM, 11, 166-169 [10.1002/cbic.200900629].
Structural features of apramycin bound at the bacterial ribosome a site as detected by NMR and CD spectroscopy
GAGGELLI, ELENA;GAGGELLI, NICOLA;VALENSIN, GIANNI
2010-01-01
Abstract
The interaction of apramycin with an RNA fragment mimicking the bacterial ribosomal A site was investigated by NMR spectroscopy, mainly by using the transferred NOE technique. The fact that only the RNA-bound antibiotic shows nonzero NOE effects, allowed us to gain structural details of bound apramycin despite the fast exchange conditions between the free and RNA-bound forms. The dissociation constant of the apramycin–RNA complex was evaluated by CD spectroscopy.
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https://hdl.handle.net/11365/3097
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