The interaction of apramycin with an RNA fragment mimicking the bacterial ribosomal A site was investigated by NMR spectroscopy, mainly by using the transferred NOE technique. The fact that only the RNA-bound antibiotic shows nonzero NOE effects, allowed us to gain structural details of bound apramycin despite the fast exchange conditions between the free and RNA-bound forms. The dissociation constant of the apramycin–RNA complex was evaluated by CD spectroscopy.

Balenci, D., D'Amelio, N., Gaggelli, E., Gaggelli, N., Cellai, L., Molteni, E., et al. (2010). Structural features of apramycin bound at the bacterial ribosome a site as detected by NMR and CD spectroscopy. CHEMBIOCHEM, 11, 166-169 [10.1002/cbic.200900629].

Structural features of apramycin bound at the bacterial ribosome a site as detected by NMR and CD spectroscopy

GAGGELLI, ELENA;GAGGELLI, NICOLA;VALENSIN, GIANNI
2010

Abstract

The interaction of apramycin with an RNA fragment mimicking the bacterial ribosomal A site was investigated by NMR spectroscopy, mainly by using the transferred NOE technique. The fact that only the RNA-bound antibiotic shows nonzero NOE effects, allowed us to gain structural details of bound apramycin despite the fast exchange conditions between the free and RNA-bound forms. The dissociation constant of the apramycin–RNA complex was evaluated by CD spectroscopy.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/3097
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo