Acute-phase serum proteins were analyzed by two-dimensional electrophoresis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of α1-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum retinol-binding protein and transthyretin were-quantitated by densitometry of silver-stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 15 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As for the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.
Bini, L., Magi, B., Marzocchi, B., Cellesi, C., Berti, B., Raggiaschi, R., et al. (1996). Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases. ELECTROPHORESIS, 17(3), 612-616 [10.1002/elps.1150170333].
Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases
Bini, Luca;Magi, Barbara;Marzocchi, Barbara;
1996-01-01
Abstract
Acute-phase serum proteins were analyzed by two-dimensional electrophoresis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of α1-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum retinol-binding protein and transthyretin were-quantitated by densitometry of silver-stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 15 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As for the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/11365/3091
Attenzione
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo