It was recently shown that fragment Lys-Ser-Pro-Val (1,2) is the major site in vivo neurofilament phosforilation. A suitable method for the preparation of this peptide was therefore studied paying particular attention to the purification step. Even thought solubility of this peptide in methyl t-butil ether does not allow the standard purification. It is possible achieve a good separation in a single step by HPLC, after a simple extraction with acidic water.

Rustici, M., Santucci, A., Lozzi, L., Pallini, V., Neri, P. (1989). Single step purification of a synthetic peptide fragment of neurofilaments by preparative high performance liquid chromatography. JOURNAL OF LIQUID CHROMATOGRAPHY, 12(9), 1579-1588 [10.1080/01483918908049527].

Single step purification of a synthetic peptide fragment of neurofilaments by preparative high performance liquid chromatography

Santucci, Annalisa;Lozzi, Luisa;Pallini, Vitaliano;Neri, Paolo
1989-01-01

Abstract

It was recently shown that fragment Lys-Ser-Pro-Val (1,2) is the major site in vivo neurofilament phosforilation. A suitable method for the preparation of this peptide was therefore studied paying particular attention to the purification step. Even thought solubility of this peptide in methyl t-butil ether does not allow the standard purification. It is possible achieve a good separation in a single step by HPLC, after a simple extraction with acidic water.
1989
Rustici, M., Santucci, A., Lozzi, L., Pallini, V., Neri, P. (1989). Single step purification of a synthetic peptide fragment of neurofilaments by preparative high performance liquid chromatography. JOURNAL OF LIQUID CHROMATOGRAPHY, 12(9), 1579-1588 [10.1080/01483918908049527].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/30804
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