It is well known that stressful stimuli change blood functions and platelet parameters are altered in humans and animals subjected to stress. We have examined the influences of behavioral stress on the morphological responses of platelets on a standard biomaterial, polyethylene (PE). Male rabbits were used, Blood was collected from the marginal vein of the ear 2 times per subject: the first sample was used as the baseline; 1 week later, the second was preceded in half of the subjects by 20 min of immobilization stress. In vitro adhesion of platelets on the PE was evaluated. The exposure of animals to stress induced a dramatic change in platelet morphology and functions on the PE: a higher degree of platelet adhesion, increased platelet spreading, and the appearance of pseudopodia, In the unstressed subjects there were no modifications of the platelets on the PE with respect to the baseline. The present experiment emphasizes for the first time the possible problems involved with the varying physiological conditions of patients before and after any biomaterial application surgery and of subjects who supply the blood for hemocompatibility tests performed on biomaterials. Therefore, in assessments of the performance of different biomaterials, the reactivity of blood factors in the patients should be considered and the test of blood compatibility should be performed with blood collected from donors in appropriate physiological conditions,

Barbucci, R., Lamponi, S., Aloisi, A.M. (1999). Effect of behavioural stress on platelet reactivity on polimeric. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 48, 9-12 [10.1002/(SICI)1097-4636(1999)48:1<9::AID-JBM3>3.0.CO;2-F].

Effect of behavioural stress on platelet reactivity on polimeric.

BARBUCCI, ROLANDO;LAMPONI, STEFANIA;ALOISI, ANNA MARIA
1999-01-01

Abstract

It is well known that stressful stimuli change blood functions and platelet parameters are altered in humans and animals subjected to stress. We have examined the influences of behavioral stress on the morphological responses of platelets on a standard biomaterial, polyethylene (PE). Male rabbits were used, Blood was collected from the marginal vein of the ear 2 times per subject: the first sample was used as the baseline; 1 week later, the second was preceded in half of the subjects by 20 min of immobilization stress. In vitro adhesion of platelets on the PE was evaluated. The exposure of animals to stress induced a dramatic change in platelet morphology and functions on the PE: a higher degree of platelet adhesion, increased platelet spreading, and the appearance of pseudopodia, In the unstressed subjects there were no modifications of the platelets on the PE with respect to the baseline. The present experiment emphasizes for the first time the possible problems involved with the varying physiological conditions of patients before and after any biomaterial application surgery and of subjects who supply the blood for hemocompatibility tests performed on biomaterials. Therefore, in assessments of the performance of different biomaterials, the reactivity of blood factors in the patients should be considered and the test of blood compatibility should be performed with blood collected from donors in appropriate physiological conditions,
Barbucci, R., Lamponi, S., Aloisi, A.M. (1999). Effect of behavioural stress on platelet reactivity on polimeric. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 48, 9-12 [10.1002/(SICI)1097-4636(1999)48:1<9::AID-JBM3>3.0.CO;2-F].
File in questo prodotto:
File Dimensione Formato  
effect beav stress 1999.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 238.69 kB
Formato Adobe PDF
238.69 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/30206
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo