Blastocyst implantation and maintenance of pregnancy involve a series of events involving endocrine and paracrine factors. In particular, hormones and cytokines appear to be very important in creating the environment essential for placenta establishment. In this study we investigated the effect of 17β-Estradiol (E2) on the secretion of the Macrophage Migration Inhibitory Factor (MIF), a pro-inflammatory cytokine highly involved in the early stages of pregnancy. By using the HTR8/SVneo cells we performed dose-response experiments with E2 concentrations ranging from 10-15 to 10-5M. We found that 10-10 and 10-13M E2 were the highest and the lowest concentrations having significant effect respectively reducing and increasing MIF levels in the culture media. Treatment with ER antagonists ICI 182 780 restored MIF levels in E2-treated samples to those of controls. These findings support interaction between hormones and cytokines in human trophoblast cells and show that MIF release is hormone receptor-dependent.

Ietta, F., Bechi, N., Bhattacharjee, J., Spagnoletti, A., Ricci, L. (2009). Hormone and cytokine interaction in human trophoblast cells. ACTA PHYSIOLOGICA, 197, 72-72.

Hormone and cytokine interaction in human trophoblast cells

IETTA F;BECHI N;BHATTACHARJEE J;SPAGNOLETTI A;RICCI L
2009-01-01

Abstract

Blastocyst implantation and maintenance of pregnancy involve a series of events involving endocrine and paracrine factors. In particular, hormones and cytokines appear to be very important in creating the environment essential for placenta establishment. In this study we investigated the effect of 17β-Estradiol (E2) on the secretion of the Macrophage Migration Inhibitory Factor (MIF), a pro-inflammatory cytokine highly involved in the early stages of pregnancy. By using the HTR8/SVneo cells we performed dose-response experiments with E2 concentrations ranging from 10-15 to 10-5M. We found that 10-10 and 10-13M E2 were the highest and the lowest concentrations having significant effect respectively reducing and increasing MIF levels in the culture media. Treatment with ER antagonists ICI 182 780 restored MIF levels in E2-treated samples to those of controls. These findings support interaction between hormones and cytokines in human trophoblast cells and show that MIF release is hormone receptor-dependent.
2009
Ietta, F., Bechi, N., Bhattacharjee, J., Spagnoletti, A., Ricci, L. (2009). Hormone and cytokine interaction in human trophoblast cells. ACTA PHYSIOLOGICA, 197, 72-72.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/29705
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo