PURPOSE: Although the retinoblastoma gene (RB/p105) has been intensely investigated as a prototype suppressor gene in humans, mutational data on the Rb family member pRb2/p130 (p130) has only recently been reported. A protective role against apoptosis has been suggested for pRb/p105, both in vitro and in vivo. However, only limited information is available on the role of pRb2/p130 in controlling apoptosis. The purpose of this study was to determine the extent of a role of this gene in the neoplasms that give the Rb family its name. METHODS: Forty-two human retinoblastomas were retrospectively examined by immunohistochemical labeling of the Rb-related proteins and the results compared with cellular kinetic characteristics: the apoptotic index (AI) and the mitotic index (MI). RESULTS: The retinoblastomas that did not express p130 showed a significantly lower AI than those that expressed p130. This result was also supported by flow cytometry on a human Saos-2 cell line that was transiently transfected with RB2/p130. The p130(-) tumors displayed a lesser degree of differentiation than the p130(+) ones. CONCLUSIONS: These observations give evidence that expression of p130 is inversely correlated with higher rates of apoptosis in human retinoblastomas and give an additional example of this regulator's role in cellular differentiation.

Bellan, C., FALCO G, D.e., Tosi, G.M., Lazzi, S., Ferrari, F., Morbini, G., et al. (2002). Missing expression of pRb2/p130 in human retinoblastomas is associated with reduced apoptosis and lesser differentiation. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 43(12), 3602-3608.

Missing expression of pRb2/p130 in human retinoblastomas is associated with reduced apoptosis and lesser differentiation.

BELLAN, CRISTIANA;TOSI, GIAN MARCO;LAZZI, STEFANO;FERRARI, FRANCESCO;TOTI, PAOLO;MANGIAVACCHI, PAOLA;CEVENINI, GABRIELE;GIORDANO, ANTONIO;LEONCINI, LORENZO;TOSI, PIERO;
2002-01-01

Abstract

PURPOSE: Although the retinoblastoma gene (RB/p105) has been intensely investigated as a prototype suppressor gene in humans, mutational data on the Rb family member pRb2/p130 (p130) has only recently been reported. A protective role against apoptosis has been suggested for pRb/p105, both in vitro and in vivo. However, only limited information is available on the role of pRb2/p130 in controlling apoptosis. The purpose of this study was to determine the extent of a role of this gene in the neoplasms that give the Rb family its name. METHODS: Forty-two human retinoblastomas were retrospectively examined by immunohistochemical labeling of the Rb-related proteins and the results compared with cellular kinetic characteristics: the apoptotic index (AI) and the mitotic index (MI). RESULTS: The retinoblastomas that did not express p130 showed a significantly lower AI than those that expressed p130. This result was also supported by flow cytometry on a human Saos-2 cell line that was transiently transfected with RB2/p130. The p130(-) tumors displayed a lesser degree of differentiation than the p130(+) ones. CONCLUSIONS: These observations give evidence that expression of p130 is inversely correlated with higher rates of apoptosis in human retinoblastomas and give an additional example of this regulator's role in cellular differentiation.
Bellan, C., FALCO G, D.e., Tosi, G.M., Lazzi, S., Ferrari, F., Morbini, G., et al. (2002). Missing expression of pRb2/p130 in human retinoblastomas is associated with reduced apoptosis and lesser differentiation. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 43(12), 3602-3608.
File in questo prodotto:
File Dimensione Formato  
7570_UPLOAD.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 401.57 kB
Formato Adobe PDF
401.57 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/28950
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo