We developed a novel surface display system based on the use of bacterial spores. A protein of the Bacillus subtilis spore coat, CotB, was found to be located on the spore surface and used as fusion partner to express the 459-amino-acid C-terminal fragment of the tetanus toxin (TTFC). Western, dot blot and fluorescent-activated cell sorting analyses were used to monitor TTFC surface expression on purified spores. We estimated that more than 1.5 x 10(3) TTFC molecules were exposed on the surface of each spore and recognized by TTFC-specific antibodies. The efficient surface presentation of the heterologous protein, together with the simple purification procedure and the high stability and safety record of B. subtilis spores, makes this spore-based display system a potentially powerful approach for surface expression of bioactive molecules.
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|Titolo:||Surface display of recombinant proteins on Bacillus subtilis spores.|
|Citazione:||Isticato, R., Cangiano, G., Tran, H.T., Ciabattini, A., Medaglini, D., Oggioni, M.R., et al. (2001). Surface display of recombinant proteins on Bacillus subtilis spores. JOURNAL OF BACTERIOLOGY, 183(21), 6294-6301.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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