A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.
Ligozzi, M., Rossolini, G.M., Tonin, E.A., Fontana, R. (1991). Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 35(3), 575-578 [10.1128/AAC.35.3.575].
Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus
Rossolini G. M.;
1991-01-01
Abstract
A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/11365/27855
Attenzione
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo