We have assessed the role of soluble P-selectin in promoting endothelial cell migration. Human endothelial cells (HUVEC) and bovine microvascular endothelial cells (CVEC) were assessed for migration in the Neuroprobe 48-well microchemotaxis chamber. Soluble P-selectin promoted a dose-dependent (0.1-10 nM) migration of both cell types, with maximal response at 10 nM, producing approximately 60% increment over basal migration. Anti-P-selectin monoclonal antibody (5 microg/ml) selectively blocked P-selectin induced migration. Fibronectin and collagen were essential to disclose the migration induced by P-selectin. It is suggested that at vascular level in the presence of modifications of the extracellular matrix milieu, the production of soluble P-selectin could contribute to angiogenesis by promoting endothelial cell migration.
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|Titolo:||Endothelial cell migration is induced by soluble P-selectin.|
|Citazione:||Morbidelli, L., Brogelli, L., Granger, H.J., & Ziche, M. (1998). Endothelial cell migration is induced by soluble P-selectin. LIFE SCIENCES, 62(1), 7-11.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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