Carbonyl compounds released during the NADPH-Fe dependent peroxidation of liver microsomal lipids and identified as 4-hydroxyalkenals (almost entirely as 4-hydroxynonenal) inhibit protein synthesis in a rabbit reticulocyte lysate. The ID50 was 0.48 mM. The inhibitory effect was reproduced by synthetic 4-hydroxynonenal. The inhibition was already evident at 1-2 min of incubation. The addition of -SH groups to the incubation medium afforded a marked protection against the inhibition of protein synthesis. The inhibitory effect seems to be due to an interaction of the carbonyl compound with -SH groups essential for the cellular protein synthetic machinery. © 1981.

Benedetti, A., Barbieri, L., Ferrali, M., Casini, A.F., Fulceri, R., Comporti, M. (1981). Inhibition of protein synthesis by carbonyl compounds (4-hydroxyalkenals) originating from the peroxidation of liver microsomal lipids. CHEMICO-BIOLOGICAL INTERACTIONS, 35(3), 331-340 [10.1016/0009-2797(81)90008-9].

Inhibition of protein synthesis by carbonyl compounds (4-hydroxyalkenals) originating from the peroxidation of liver microsomal lipids

Ferrali, M.;Fulceri, R.;Comporti, M.
1981-01-01

Abstract

Carbonyl compounds released during the NADPH-Fe dependent peroxidation of liver microsomal lipids and identified as 4-hydroxyalkenals (almost entirely as 4-hydroxynonenal) inhibit protein synthesis in a rabbit reticulocyte lysate. The ID50 was 0.48 mM. The inhibitory effect was reproduced by synthetic 4-hydroxynonenal. The inhibition was already evident at 1-2 min of incubation. The addition of -SH groups to the incubation medium afforded a marked protection against the inhibition of protein synthesis. The inhibitory effect seems to be due to an interaction of the carbonyl compound with -SH groups essential for the cellular protein synthetic machinery. © 1981.
Benedetti, A., Barbieri, L., Ferrali, M., Casini, A.F., Fulceri, R., Comporti, M. (1981). Inhibition of protein synthesis by carbonyl compounds (4-hydroxyalkenals) originating from the peroxidation of liver microsomal lipids. CHEMICO-BIOLOGICAL INTERACTIONS, 35(3), 331-340 [10.1016/0009-2797(81)90008-9].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/27383
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