Here, a three-dimensional model based on fragments of human de-epidermized dermis (DED) is prepared in order to study the performance of a microperforated, hyaluronan-based membrane as a carrier of cultured epidermal cells. Hyaluronic acid is, in fact, considered to be an optimal biomaterial allowing proliferation of both keratinocytes and melanocytes, and it is already used for clinical aims. The carrier with subconfluent human epidermal cultures is positioned onto the DED and kept in culture until a new epidermis is formed. This model system allowed to study the migration and growth of human epidermal cells from the carrier, resembling 'in vivo' re-epithelization. Copyright (C) 1999 Elsevier Science Ltd. Here, a three-dimensional model based on fragments of human de-epidermized dermis (DED) is prepared in order to study the performance of a microperforated, hyaluronan-based membrane as a carrier of cultured epidermal cells. Hyaluronic acid is, in fact, considered to be an optimal biomaterial allowing proliferation of both keratinocytes and melanocytes, and it is already used for clinical aims. The carrier with subconfluent human epidermal cultures is positioned onto the DED and kept in culture until a new epidermis is formed. This model system allowed to study the migration and growth of human epidermal cells from the carrier, resembling 'in vivo' re-epithelization.

Pianigiani, E., Andreassi, A., Taddeucci, P., Alessandrini, C., Fimiani, M., Andreassi, L. (2000). A new model for studying differentiation and growth of epidermal cultures on a hyluronan-based carrier. BIOMATERIALS, 20(18), 1689-1694 [10.1016/S0142-9612(99)00056-3].

A new model for studying differentiation and growth of epidermal cultures on a hyluronan-based carrier

Taddeucci, P.;Fimiani, M.;
2000-01-01

Abstract

Here, a three-dimensional model based on fragments of human de-epidermized dermis (DED) is prepared in order to study the performance of a microperforated, hyaluronan-based membrane as a carrier of cultured epidermal cells. Hyaluronic acid is, in fact, considered to be an optimal biomaterial allowing proliferation of both keratinocytes and melanocytes, and it is already used for clinical aims. The carrier with subconfluent human epidermal cultures is positioned onto the DED and kept in culture until a new epidermis is formed. This model system allowed to study the migration and growth of human epidermal cells from the carrier, resembling 'in vivo' re-epithelization. Copyright (C) 1999 Elsevier Science Ltd. Here, a three-dimensional model based on fragments of human de-epidermized dermis (DED) is prepared in order to study the performance of a microperforated, hyaluronan-based membrane as a carrier of cultured epidermal cells. Hyaluronic acid is, in fact, considered to be an optimal biomaterial allowing proliferation of both keratinocytes and melanocytes, and it is already used for clinical aims. The carrier with subconfluent human epidermal cultures is positioned onto the DED and kept in culture until a new epidermis is formed. This model system allowed to study the migration and growth of human epidermal cells from the carrier, resembling 'in vivo' re-epithelization.
2000
Pianigiani, E., Andreassi, A., Taddeucci, P., Alessandrini, C., Fimiani, M., Andreassi, L. (2000). A new model for studying differentiation and growth of epidermal cultures on a hyluronan-based carrier. BIOMATERIALS, 20(18), 1689-1694 [10.1016/S0142-9612(99)00056-3].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/2736
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