This study was undertaken in order to study the effects of short-term exposure of the follicle-associated epithelium (FAE) of rabbit Peyer's patches to a non-intestinal, Gram-positive bacterium. Isolated ileal loops, each containing one Peyer's patch (PP), were stimulated for short periods of time (30 and 60 min) with Streptococcus pneumoniae R36a, a micro-organism normally not present in the intestinal area. Samples from antigen-stimulated and control Peyer's patches were analysed by light (LM), transmission electron (TEM), and scanning electron microscopy (SEM). Stimulation with living pneumococci induced dramatic changes in FAE architecture and morphology. A massive passage of cells from lymphoid tissue to the FAE was rapidly detectable, accompanied by alterations of the FAE surface, with a marked increase of M-cell area. Furthermore, TEM analysis revealed that M cells were able to internalize living pneumococci. S. pneumoniae R36a is a valid experimental model for the further study of the unique antigen sampling function which characterizes the highly specialized FAE in Peyer's patches.

Borghesi, C., Regoli, M., Bertelli, E., & Nicoletti, C. (1996). Modifications of the follicle-associated epithelium by short-term exposure to a non-intestinal bacterium. JOURNAL OF PATHOLOGY, 180(3), 326-332 [10.1002/(SICI)1096-9896(199611)180:3<326::AID-PATH656>3.0.CO;2-6].

Modifications of the follicle-associated epithelium by short-term exposure to a non-intestinal bacterium

REGOLI, MARI';BERTELLI, EUGENIO;
1996

Abstract

This study was undertaken in order to study the effects of short-term exposure of the follicle-associated epithelium (FAE) of rabbit Peyer's patches to a non-intestinal, Gram-positive bacterium. Isolated ileal loops, each containing one Peyer's patch (PP), were stimulated for short periods of time (30 and 60 min) with Streptococcus pneumoniae R36a, a micro-organism normally not present in the intestinal area. Samples from antigen-stimulated and control Peyer's patches were analysed by light (LM), transmission electron (TEM), and scanning electron microscopy (SEM). Stimulation with living pneumococci induced dramatic changes in FAE architecture and morphology. A massive passage of cells from lymphoid tissue to the FAE was rapidly detectable, accompanied by alterations of the FAE surface, with a marked increase of M-cell area. Furthermore, TEM analysis revealed that M cells were able to internalize living pneumococci. S. pneumoniae R36a is a valid experimental model for the further study of the unique antigen sampling function which characterizes the highly specialized FAE in Peyer's patches.
Borghesi, C., Regoli, M., Bertelli, E., & Nicoletti, C. (1996). Modifications of the follicle-associated epithelium by short-term exposure to a non-intestinal bacterium. JOURNAL OF PATHOLOGY, 180(3), 326-332 [10.1002/(SICI)1096-9896(199611)180:3<326::AID-PATH656>3.0.CO;2-6].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/27317
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