Abstract In this study we showed that Rottlerin (also called Kamala or Mallotoxin), a natural product purified from Mallotus phillippinensis, is a potent suppressor of human keratinocytes (HaCaT cell line) proliferation. Following Rottlerin treatment, Thymidine incorporation into DNA and re-epithelialisation in a scratch wound model was decreased. At the molecular level, Rottlerin hampered the NFkB activation process, causing loss of cyclin D1 and promoting, in a PKCdelta-dependent pathway, ERK activation, which, in turn induced the cell cycle inhibitor p21 Cip1/Kip1. The NFkB-dependent drop in cyclin D1, along with the PKCdelta/ERK-dependent induction of p21 Cip1/Kip1, is responsible for growth arrest. These results open the way to further investigation on the Rottlerin therapeutic potential against keratinocyte hyper-proliferative disorders.

Valacchi, G., Pecorelli, A., Mencarelli, M., Carbotti, P., Fortino, V., Muscettola, M.M., et al. (2009). Rottlerin: a multifaced regulator of keratinocyte cell cycle. EXPERIMENTAL DERMATOLOGY, 18(6), 516-521 [10.1111/j.1600-0625.2008.00816.x].

Rottlerin: a multifaced regulator of keratinocyte cell cycle.

MUSCETTOLA, MARIA MICHELA;MAIOLI, EMANUELA
2009-01-01

Abstract

Abstract In this study we showed that Rottlerin (also called Kamala or Mallotoxin), a natural product purified from Mallotus phillippinensis, is a potent suppressor of human keratinocytes (HaCaT cell line) proliferation. Following Rottlerin treatment, Thymidine incorporation into DNA and re-epithelialisation in a scratch wound model was decreased. At the molecular level, Rottlerin hampered the NFkB activation process, causing loss of cyclin D1 and promoting, in a PKCdelta-dependent pathway, ERK activation, which, in turn induced the cell cycle inhibitor p21 Cip1/Kip1. The NFkB-dependent drop in cyclin D1, along with the PKCdelta/ERK-dependent induction of p21 Cip1/Kip1, is responsible for growth arrest. These results open the way to further investigation on the Rottlerin therapeutic potential against keratinocyte hyper-proliferative disorders.
2009
Valacchi, G., Pecorelli, A., Mencarelli, M., Carbotti, P., Fortino, V., Muscettola, M.M., et al. (2009). Rottlerin: a multifaced regulator of keratinocyte cell cycle. EXPERIMENTAL DERMATOLOGY, 18(6), 516-521 [10.1111/j.1600-0625.2008.00816.x].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/27264
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