The current study describes a statistically significant increase in macrophages (CD68-positive cells) in the decidua of preeclamptic patients. To elucidate the regulation of this monocyte infiltration, expression of monocyte chemoattractant protein-1 (MCP-1) was assessed in leukocyte-free first trimester decidual cells. Confluent decidual cells were primed for 7 days in either estradiol or estradiol plus medroxyprogesterone acetate to mimic the decidualizing steroidal milieu of the luteal phase and early pregnancy. The medium was exchanged for a serum-free defined medium containing corresponding steroids / tumor necrosis factor (TNF)- or interleukin (IL)-1. After 24 hours, enzyme-linked immunosorbent assay measurements indicated that the addition of medroxyprogesterone acetate did not affect MCP-1 output, whereas 10 ng/ml of TNF- or IL-1 increased output by 83.5-fold 20.6 and 103.1-fold 14.7, respectively (mean SEM, n 8, P < 0.05). Concentration-response comparisons revealed that even 0.01 ng/ml of TNF- or IL-1 elevated MCP-1 output by more than 15-fold. Western blotting confirmed the enzymelinked immunosorbent assay results, and quantitative reverse transcriptase-polymerase chain reaction confirmed corresponding effects on MCP-1 mRNA levels. The current study demonstrates that TNF- and IL-1 enhance MCP-1 in first trimester decidua. This finding suggests a mechanism by which recruitment of excess macrophages to the decidua impairs endovascular trophoblast invasion, the primary placental defect of preeclampsia.

Lockwood, C.j., Matta, P., Krikun, G., Koopman, L.a., Masch, R., Toti, P., et al. (2006). Regulation of monocyte chemoattractant protein-1 expression by tumor necrosis factor-alpha and interleukin-1beta in first trimester human decidual cells: implications for preeclampsia. THE AMERICAN JOURNAL OF PATHOLOGY, 168, 445-452 [10.2353/ajpath.2006.050082].

Regulation of monocyte chemoattractant protein-1 expression by tumor necrosis factor-alpha and interleukin-1beta in first trimester human decidual cells: implications for preeclampsia.

TOTI, PAOLO;
2006

Abstract

The current study describes a statistically significant increase in macrophages (CD68-positive cells) in the decidua of preeclamptic patients. To elucidate the regulation of this monocyte infiltration, expression of monocyte chemoattractant protein-1 (MCP-1) was assessed in leukocyte-free first trimester decidual cells. Confluent decidual cells were primed for 7 days in either estradiol or estradiol plus medroxyprogesterone acetate to mimic the decidualizing steroidal milieu of the luteal phase and early pregnancy. The medium was exchanged for a serum-free defined medium containing corresponding steroids / tumor necrosis factor (TNF)- or interleukin (IL)-1. After 24 hours, enzyme-linked immunosorbent assay measurements indicated that the addition of medroxyprogesterone acetate did not affect MCP-1 output, whereas 10 ng/ml of TNF- or IL-1 increased output by 83.5-fold 20.6 and 103.1-fold 14.7, respectively (mean SEM, n 8, P < 0.05). Concentration-response comparisons revealed that even 0.01 ng/ml of TNF- or IL-1 elevated MCP-1 output by more than 15-fold. Western blotting confirmed the enzymelinked immunosorbent assay results, and quantitative reverse transcriptase-polymerase chain reaction confirmed corresponding effects on MCP-1 mRNA levels. The current study demonstrates that TNF- and IL-1 enhance MCP-1 in first trimester decidua. This finding suggests a mechanism by which recruitment of excess macrophages to the decidua impairs endovascular trophoblast invasion, the primary placental defect of preeclampsia.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/26480
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