We analyzed functional activity of the antimicrobial peptide M6 in vitro and in vivo. The peptide was identified by our group by phage library selection, rational modification and synthesis in a tetrabranched form (Pini et al., Antimicrob. Agents Chemother. 2005; 49: 2665–72). We found that it binds lipopolysaccharide, causes perforation of cell membranes without destroying external cell morphology and strongly binds DNA. The latter feature suggests that it could inhibit metabolic pathways, blocking DNA replication and/or transcription. We also observed that M6 does not stimulate humoral immune response when repeatedly administered to animals. We also analyzed M6 toxicity when administered to animals by intraperitoneal or by intravenous injection, determining a preliminary LD50 (125 and 37.5 mg/kg, respectively), which suggested that M6 could be used in vivo. These features make the antimicrobial branched peptide M6 a promising candidate for the development of a new antibacterial drug.

Pini, A., Giuliani, A., Falciani, C., Fabbrini, M., Pileri, S., Lelli, B., et al. (2007). Characterization of the branched antimicrobial peptide M6 by analyzing its mechanism of action and in vivo toxicity. JOURNAL OF PEPTIDE SCIENCE, 13(6), 393-399 [10.1002/psc.858].

Characterization of the branched antimicrobial peptide M6 by analyzing its mechanism of action and in vivo toxicity.

Pini, Alessandro;GIULIANI, ANDREA;Falciani, Chiara;Fabbrini, Monica;Pileri, Silvia;Lelli, Barbara;Bracci, Luisa
2007-01-01

Abstract

We analyzed functional activity of the antimicrobial peptide M6 in vitro and in vivo. The peptide was identified by our group by phage library selection, rational modification and synthesis in a tetrabranched form (Pini et al., Antimicrob. Agents Chemother. 2005; 49: 2665–72). We found that it binds lipopolysaccharide, causes perforation of cell membranes without destroying external cell morphology and strongly binds DNA. The latter feature suggests that it could inhibit metabolic pathways, blocking DNA replication and/or transcription. We also observed that M6 does not stimulate humoral immune response when repeatedly administered to animals. We also analyzed M6 toxicity when administered to animals by intraperitoneal or by intravenous injection, determining a preliminary LD50 (125 and 37.5 mg/kg, respectively), which suggested that M6 could be used in vivo. These features make the antimicrobial branched peptide M6 a promising candidate for the development of a new antibacterial drug.
Pini, A., Giuliani, A., Falciani, C., Fabbrini, M., Pileri, S., Lelli, B., et al. (2007). Characterization of the branched antimicrobial peptide M6 by analyzing its mechanism of action and in vivo toxicity. JOURNAL OF PEPTIDE SCIENCE, 13(6), 393-399 [10.1002/psc.858].
File in questo prodotto:
File Dimensione Formato  
Articolo Pini et al., 2007 JPS.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 457.05 kB
Formato Adobe PDF
457.05 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/26347
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo