The NaK channel is a cation selective channel with similar permeability for K(+) and Na(+). The available crystallographic structure of wild-type (WT) NaK is usually associated with a conductive state of the channel. Here, potential of mean force for complete conduction events of Na(+) and K(+) ions through NaK show that: i), large energy barriers prevent the passage of ions through the WT NaK structure, ii), the barriers are correlated to the presence of a hydrogen bond between Asp-66 and Asn-68, and iii), the structure of NaK mutated to mimic cyclic nucleotide-gated channels conducts Na(+) and K(+). These results support the hypothesis that the filter of cation selective channels can adopt at least two different structures: a conductive one, represented by the x-ray structures of the NaK-CNG chimeras, and a closed one, represented by the x-ray structures of the WT NaK.

Furini, S., & Domene, C. (2011). Gating at the Selectivity Filter of Ion Channels that Conduct Na(+) and K(+) Ions. BIOPHYSICAL JOURNAL, 101(7), 1623-1631 [10.1016/j.bpj.2011.08.035].

Gating at the Selectivity Filter of Ion Channels that Conduct Na(+) and K(+) Ions

FURINI, SIMONE;
2011

Abstract

The NaK channel is a cation selective channel with similar permeability for K(+) and Na(+). The available crystallographic structure of wild-type (WT) NaK is usually associated with a conductive state of the channel. Here, potential of mean force for complete conduction events of Na(+) and K(+) ions through NaK show that: i), large energy barriers prevent the passage of ions through the WT NaK structure, ii), the barriers are correlated to the presence of a hydrogen bond between Asp-66 and Asn-68, and iii), the structure of NaK mutated to mimic cyclic nucleotide-gated channels conducts Na(+) and K(+). These results support the hypothesis that the filter of cation selective channels can adopt at least two different structures: a conductive one, represented by the x-ray structures of the NaK-CNG chimeras, and a closed one, represented by the x-ray structures of the WT NaK.
Furini, S., & Domene, C. (2011). Gating at the Selectivity Filter of Ion Channels that Conduct Na(+) and K(+) Ions. BIOPHYSICAL JOURNAL, 101(7), 1623-1631 [10.1016/j.bpj.2011.08.035].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/26233
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