The possibility of ameliorating bone healing of implanted bone allografts is a field of great interest. Early vascular invasion is a key factor in bone allograft incorporation. It is well known that copper ions (Cu2+) show a proangiogenic action favouring the development of new vessels. In this work a hyaluronan based 50% hydrogel (Hyal-50%) was enriched with (Cu2+) and its proangiogenic activity was evaluated. Fifteen Sprague Dawley female rats were submitted to the subcutaneous implantation of Hyal-50%, freeze-dried bone allografts, Hyal-50%-Cu2+, freeze-dried bone allografts plus Hyal-50% and freeze-dried bone allografts plus Hyal-50%-Cu2+. One month later, histomorphometric analysis evidenced the presence of a fibrous-reactive capsule around all specimens showing significant differences among groups (po<0.0005). The highest thickness of the fibrous capsule was found around the freeze-dried bone implants (po<0.05); as well as the Hyal 50%-Cu2+ plus freeze-dried bone (15.2%, po<0.05) and Hyal 50% plus freeze-dried bone (21.4%, po<0.0005) implants showed a significant higher thickness compared with Hyal 50% and Hyal-50% Cu2+. Statistical analysis showed a significant (po<0.01) higher vascular density in Hyal- 50%-Cu2+ and Hyal-50%- Cu2+ plus freeze-dried bone group when compared to other groups. The present preliminary results suggest the advantages offered by the combined use of a well-known biocompatible and tissue healing promoting material (Hyal-50%) and a new technique that consists of stimulating tissue vascularization using Cu2+ and that bone allograft incorporation may benefit from this technology.

G., G., P., T., P., F., R., G., Barbucci, R., Leone, G. (2005). Blood vessel formation after soft tissue implantation of hyaluronan based hydrogel supplemented with copper ions. BIOMATERIALS, 26, 3001-3008 [10.1016/j.biomaterials.2004.08.027].

Blood vessel formation after soft tissue implantation of hyaluronan based hydrogel supplemented with copper ions

BARBUCCI, ROLANDO;LEONE, GEMMA
2005-01-01

Abstract

The possibility of ameliorating bone healing of implanted bone allografts is a field of great interest. Early vascular invasion is a key factor in bone allograft incorporation. It is well known that copper ions (Cu2+) show a proangiogenic action favouring the development of new vessels. In this work a hyaluronan based 50% hydrogel (Hyal-50%) was enriched with (Cu2+) and its proangiogenic activity was evaluated. Fifteen Sprague Dawley female rats were submitted to the subcutaneous implantation of Hyal-50%, freeze-dried bone allografts, Hyal-50%-Cu2+, freeze-dried bone allografts plus Hyal-50% and freeze-dried bone allografts plus Hyal-50%-Cu2+. One month later, histomorphometric analysis evidenced the presence of a fibrous-reactive capsule around all specimens showing significant differences among groups (po<0.0005). The highest thickness of the fibrous capsule was found around the freeze-dried bone implants (po<0.05); as well as the Hyal 50%-Cu2+ plus freeze-dried bone (15.2%, po<0.05) and Hyal 50% plus freeze-dried bone (21.4%, po<0.0005) implants showed a significant higher thickness compared with Hyal 50% and Hyal-50% Cu2+. Statistical analysis showed a significant (po<0.01) higher vascular density in Hyal- 50%-Cu2+ and Hyal-50%- Cu2+ plus freeze-dried bone group when compared to other groups. The present preliminary results suggest the advantages offered by the combined use of a well-known biocompatible and tissue healing promoting material (Hyal-50%) and a new technique that consists of stimulating tissue vascularization using Cu2+ and that bone allograft incorporation may benefit from this technology.
G., G., P., T., P., F., R., G., Barbucci, R., Leone, G. (2005). Blood vessel formation after soft tissue implantation of hyaluronan based hydrogel supplemented with copper ions. BIOMATERIALS, 26, 3001-3008 [10.1016/j.biomaterials.2004.08.027].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/25875
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