Plasma human immunodeficiency virus (HIV) type 1 RNA load is the reference marker for response to antiretroviral therapy. To compare peripheral blood mononuclear cell (PBMC)-associated and plasma HIV-1 RNA response to treatment, HIV-1 RNA was quantified by reverse transcription-competitive polymerase chain reaction in 20 patients at 0, 12, and 24 weeks following addition of saquinavir to their treatment regimens. HIV-1 RNA was undetectable in 15 plasma samples but in only 2 PBMC samples (P=.002) and CD4 cell counts correlated more with PBMC than with plasma HIV-1 RNA load. Changes in HIV-1 RNA load in PBMC and in plasma were correlated, and the decrease was higher in plasma than in PBMC at weeks 12 (P=.002) and 24 (P=.017). Moreover, PBMC, but not plasma HIV-1 load, at week 12 was predictive of HIV-1 RNA levels at week 24 in both plasma (P=.004) and PBMC (P<. 001). Thus, measurement of PBMC HIV-1 RNA may be useful during antiretroviral therapy.

Romano, L., Venturi, G., Catucci, M., DE MILITO, A., Valensin, P.E., Zazzi, M. (1999). Evaluation of cell-free and cell-associated peripheral blood HIV-1 RNA response to antiretroviral therapy. THE JOURNAL OF INFECTIOUS DISEASES, 179, 361-366.

Evaluation of cell-free and cell-associated peripheral blood HIV-1 RNA response to antiretroviral therapy

ROMANO, LAURA;VALENSIN, PIER EGISTO;ZAZZI, MAURIZIO
1999-01-01

Abstract

Plasma human immunodeficiency virus (HIV) type 1 RNA load is the reference marker for response to antiretroviral therapy. To compare peripheral blood mononuclear cell (PBMC)-associated and plasma HIV-1 RNA response to treatment, HIV-1 RNA was quantified by reverse transcription-competitive polymerase chain reaction in 20 patients at 0, 12, and 24 weeks following addition of saquinavir to their treatment regimens. HIV-1 RNA was undetectable in 15 plasma samples but in only 2 PBMC samples (P=.002) and CD4 cell counts correlated more with PBMC than with plasma HIV-1 RNA load. Changes in HIV-1 RNA load in PBMC and in plasma were correlated, and the decrease was higher in plasma than in PBMC at weeks 12 (P=.002) and 24 (P=.017). Moreover, PBMC, but not plasma HIV-1 load, at week 12 was predictive of HIV-1 RNA levels at week 24 in both plasma (P=.004) and PBMC (P<. 001). Thus, measurement of PBMC HIV-1 RNA may be useful during antiretroviral therapy.
Romano, L., Venturi, G., Catucci, M., DE MILITO, A., Valensin, P.E., Zazzi, M. (1999). Evaluation of cell-free and cell-associated peripheral blood HIV-1 RNA response to antiretroviral therapy. THE JOURNAL OF INFECTIOUS DISEASES, 179, 361-366.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/25738
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