The lethal and oedema toxins produced by Bacillus anthracis, the aetiological agent of anthrax, are made by association of protective antigen with lethal and oedema factors and play a major role in the pathogenesis of anthrax. In the present paper, we describe the production of peptide-based specific inhibitors in branched form which inhibit the interaction of protective antigen with lethal and oedema factors and neutralize anthrax toxins in vitro and in vivo. Anti-protective antigen peptides were selected from a phage library by competitive panning with lethal factor. Selected 12-mer peptides were synthesized in tetra-branched form and were systematically modified to obtain peptides with higher affinity and inhibitory efficiency

Pini, A., Runci, Y., Falciani, C., Lelli, B., Brunetti, J., Pileri, S., et al. (2006). Stable peptide inhibitors prevent binding of lethal and oedema factors to protective antigen and neutralize anthrax toxin in vivo. BIOCHEMICAL JOURNAL, 395(1), 157-163 [10.1042/BJ20051747].

Stable peptide inhibitors prevent binding of lethal and oedema factors to protective antigen and neutralize anthrax toxin in vivo

PINI, A.;RUNCI, Y.;FALCIANI, C.;BRUNETTI, J.;LOZZI, L.;NICCOLAI, N.;BRACCI, L.
2006-01-01

Abstract

The lethal and oedema toxins produced by Bacillus anthracis, the aetiological agent of anthrax, are made by association of protective antigen with lethal and oedema factors and play a major role in the pathogenesis of anthrax. In the present paper, we describe the production of peptide-based specific inhibitors in branched form which inhibit the interaction of protective antigen with lethal and oedema factors and neutralize anthrax toxins in vitro and in vivo. Anti-protective antigen peptides were selected from a phage library by competitive panning with lethal factor. Selected 12-mer peptides were synthesized in tetra-branched form and were systematically modified to obtain peptides with higher affinity and inhibitory efficiency
2006
Pini, A., Runci, Y., Falciani, C., Lelli, B., Brunetti, J., Pileri, S., et al. (2006). Stable peptide inhibitors prevent binding of lethal and oedema factors to protective antigen and neutralize anthrax toxin in vivo. BIOCHEMICAL JOURNAL, 395(1), 157-163 [10.1042/BJ20051747].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/25682