Seventeen S. aureus clinical isolates, collected from an Intensive Care Unit (ICU) during a seven-month period were analyzed to investigate their antimicrobial susceptibility and clonal diversity. Eleven isolates (65%) were found to be resistant to methicillin (MRSA). Pulsed-field gel electrophoresis (PFGE) profiles of genomic DNAs, and analysis of the polymorphisms of the variable regions of the protein A (spa) and coagulase (coa) genes revealed a lower clonal heterogeneity among MRSA than among methicillin-susceptible isolates (MSSA). Two of the MRSA clones were repeatedly isolated in different patients, within a variable period of time, suggesting the presence in the ward of a resident, endemic and multi-drug resistant MRSA population. Our results also emphasize the lower discriminatory power of spa and coa typing compared with PFGE typing.

Zanelli, G., Pollini, S., Sansoni, A., Cresti, S., Pilli, E., Rossolini, G.M., et al. (2004). Molecular typing of Staphylococcus aureus isolates from an intensive care unit. NEW MICROBIOLOGICA, 27, 293-299.

Molecular typing of Staphylococcus aureus isolates from an intensive care unit.

ZANELLI, GIACOMO;POLLINI, SIMONA;CRESTI, STEFANIA;ROSSOLINI, GIAN MARIA;CELLESI, CARLA
2004

Abstract

Seventeen S. aureus clinical isolates, collected from an Intensive Care Unit (ICU) during a seven-month period were analyzed to investigate their antimicrobial susceptibility and clonal diversity. Eleven isolates (65%) were found to be resistant to methicillin (MRSA). Pulsed-field gel electrophoresis (PFGE) profiles of genomic DNAs, and analysis of the polymorphisms of the variable regions of the protein A (spa) and coagulase (coa) genes revealed a lower clonal heterogeneity among MRSA than among methicillin-susceptible isolates (MSSA). Two of the MRSA clones were repeatedly isolated in different patients, within a variable period of time, suggesting the presence in the ward of a resident, endemic and multi-drug resistant MRSA population. Our results also emphasize the lower discriminatory power of spa and coa typing compared with PFGE typing.
Zanelli, G., Pollini, S., Sansoni, A., Cresti, S., Pilli, E., Rossolini, G.M., et al. (2004). Molecular typing of Staphylococcus aureus isolates from an intensive care unit. NEW MICROBIOLOGICA, 27, 293-299.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/25673
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