Alzheimer's disease is a fatal neurodegenerative disorder involving the abnormal accumulation and deposition of peptides (amyloid-beta, A beta) derived from the amyloid precursor protein. Here, we present the structure and the Zn(2+) binding sites of human and rat A beta(1 -28) fragments in water/sodium dodecyl sulfate (SDS) micelles by using (1)H NMR spectroscopy. The chemical shift variations measured after Zn(2+) addition at T > 310 K allowed us to assign the binding donor atoms in both rat and human zinc complexes. The Asp-1 amine, His-6 N delta, Glu-11 COO(-), and His-13 N epsilon of rat A beta(28) all enter the metal coordination sphere, while His-6 N delta, His-13, His-14 N epsilon, Asp-1 amine, and/or Glu-11 COO(-) are all bound to Zn(2+) in the case of human A beta(28). Finally, a comparison between the rat and human binding abilities was discussed.
Gaggelli, E., JANICKA KLOS, A., Jankowska, E., Kozlowski, H., Migliorini, C., Molteni, E., et al. (2008). NMR Studies of the Zn2+ Interactions with Rat and Human β-Amyloid (1-28) Peptides in Water-Micelle Environment. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL, 112(1), 100-109 [10.1021/jp075168m].
NMR Studies of the Zn2+ Interactions with Rat and Human β-Amyloid (1-28) Peptides in Water-Micelle Environment
GAGGELLI, ELENA;MIGLIORINI, CATERINA;VALENSIN, DANIELA;VALENSIN, GIANNI;
2008-01-01
Abstract
Alzheimer's disease is a fatal neurodegenerative disorder involving the abnormal accumulation and deposition of peptides (amyloid-beta, A beta) derived from the amyloid precursor protein. Here, we present the structure and the Zn(2+) binding sites of human and rat A beta(1 -28) fragments in water/sodium dodecyl sulfate (SDS) micelles by using (1)H NMR spectroscopy. The chemical shift variations measured after Zn(2+) addition at T > 310 K allowed us to assign the binding donor atoms in both rat and human zinc complexes. The Asp-1 amine, His-6 N delta, Glu-11 COO(-), and His-13 N epsilon of rat A beta(28) all enter the metal coordination sphere, while His-6 N delta, His-13, His-14 N epsilon, Asp-1 amine, and/or Glu-11 COO(-) are all bound to Zn(2+) in the case of human A beta(28). Finally, a comparison between the rat and human binding abilities was discussed.File | Dimensione | Formato | |
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https://hdl.handle.net/11365/25607
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