Non-pathogenic Gram-positive oral commensal bacteria expressing recombinant fusion proteins on their cell surface have been successfully used to raise both a mucosal and a systemic immune response to foreign antigens while colonizing the oropharynx. In this system, fusion-protein vaccines are delivered and anchored to the surface of a commensal, which occupies the mucosal niche invaded by a particular pathogen. Surface expression of these foreign proteins is achieved by exploiting the common mechanism employed by Gram-positive bacteria for translocating and anchoring proteins to the cell surface. The process offers a safe alternative to the use of engineered pathogens as live vaccine delivery vehicles.

Fischetti, V.A., Medaglini, D., Oggioni, M., & Pozzi, G. (1993). Expression of foreign proteins on gram-positive commensal bacteria for mucosal vaccine delivery. CURRENT OPINION IN BIOTECHNOLOGY, 4(5), 603-610 [10.1016/0958-1669(93)90084-A].

Expression of foreign proteins on gram-positive commensal bacteria for mucosal vaccine delivery.

MEDAGLINI, DONATA;POZZI, GIANNI
1993

Abstract

Non-pathogenic Gram-positive oral commensal bacteria expressing recombinant fusion proteins on their cell surface have been successfully used to raise both a mucosal and a systemic immune response to foreign antigens while colonizing the oropharynx. In this system, fusion-protein vaccines are delivered and anchored to the surface of a commensal, which occupies the mucosal niche invaded by a particular pathogen. Surface expression of these foreign proteins is achieved by exploiting the common mechanism employed by Gram-positive bacteria for translocating and anchoring proteins to the cell surface. The process offers a safe alternative to the use of engineered pathogens as live vaccine delivery vehicles.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/2447
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