Laccase, isolated from Cerrena unicolor, is able to transform 3-amino-4-hydroxybenzensulfonic acid into a water soluble phenoxazine dye with an extinction coefficient (ε) of 8600M−1 cm−1. The dye has been characterized using a variety of different analytic and spectroscopic techniques like UV–vis spectroscopy, HPLC (High Performance Liquid Chromatography), ESI/MS (Electrospray Ionization Mass Spectrometry) and the following NMR experiments: 1H, 13C, TOCSY (Total Correlation Spectroscopy), HSQC (Heteronuclear SingleQuantumCoherence),HMBC(Heteronuclear Multiple Bond Coherence) showing the structure of 2-amino-3-oxo-3H-phenoxazine-8-sulfonic acid. The advantages of the presented biocatalytic system, in alignment with chemical system to obtain Curie 22, are eco-sustainability and one step performance.
Forte, S., Polak, J., Valensin, D., Taddei, M., Basosi, R., Vanhulle, S., et al. (2010). Synthesis and structural characterization of a novel phenoxazinone dye by use of a fungal laccase. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 63, 116-120 [10.1016/j.molcatb.2009.12.018].
Synthesis and structural characterization of a novel phenoxazinone dye by use of a fungal laccase
FORTE, STEFANIA;VALENSIN, DANIELA;TADDEI, MAURIZIO;BASOSI, RICCARDO;POGNI, REBECCA
2010-01-01
Abstract
Laccase, isolated from Cerrena unicolor, is able to transform 3-amino-4-hydroxybenzensulfonic acid into a water soluble phenoxazine dye with an extinction coefficient (ε) of 8600M−1 cm−1. The dye has been characterized using a variety of different analytic and spectroscopic techniques like UV–vis spectroscopy, HPLC (High Performance Liquid Chromatography), ESI/MS (Electrospray Ionization Mass Spectrometry) and the following NMR experiments: 1H, 13C, TOCSY (Total Correlation Spectroscopy), HSQC (Heteronuclear SingleQuantumCoherence),HMBC(Heteronuclear Multiple Bond Coherence) showing the structure of 2-amino-3-oxo-3H-phenoxazine-8-sulfonic acid. The advantages of the presented biocatalytic system, in alignment with chemical system to obtain Curie 22, are eco-sustainability and one step performance.
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https://hdl.handle.net/11365/24376
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