The effects of different concentrations (10-5, 5 x 10-5 and 10-4 M) of copper bromide on spore germination, growth and ultrastructure were investigated in Polypodium cambricum L. gametophytes. The inhibitory effect of Cu was observed in spores cultured on medium supplemented with 10-4 M CuBr2: germination occurred about 40 days after sowing and was only 25%. Concentrations of 5 x 10-5 and 10-4 M CuBr2 induced changes in gametophyte development, possibly by re-orientation of growth. Gametophytes treated with 10-5 and 5x 10-5 M CuBr2 took up and accumulated a large amount of copper and ultrastructural observations showed that cytoplasmic damage was limited to twisted swollen thylakoids. The ultrastructure of gametophytes treated with 10-4 M CuBr2 showed absence of a vacuolar compartment. The present observations suggest that P. cambricum gametophytes could be a suitable material for studying physiological and molecular alterations induced by excess copper.

Muccifora, S. (2008). Effects of copper on spore germination, growth and ultrastructure of Polypodium cambricum L. gametophytes. ENVIRONMENTAL POLLUTION, 153(2), 369-375 [10.1016/j.envpol.2007.08.012].

Effects of copper on spore germination, growth and ultrastructure of Polypodium cambricum L. gametophytes

Muccifora, Simonetta
2008-01-01

Abstract

The effects of different concentrations (10-5, 5 x 10-5 and 10-4 M) of copper bromide on spore germination, growth and ultrastructure were investigated in Polypodium cambricum L. gametophytes. The inhibitory effect of Cu was observed in spores cultured on medium supplemented with 10-4 M CuBr2: germination occurred about 40 days after sowing and was only 25%. Concentrations of 5 x 10-5 and 10-4 M CuBr2 induced changes in gametophyte development, possibly by re-orientation of growth. Gametophytes treated with 10-5 and 5x 10-5 M CuBr2 took up and accumulated a large amount of copper and ultrastructural observations showed that cytoplasmic damage was limited to twisted swollen thylakoids. The ultrastructure of gametophytes treated with 10-4 M CuBr2 showed absence of a vacuolar compartment. The present observations suggest that P. cambricum gametophytes could be a suitable material for studying physiological and molecular alterations induced by excess copper.
2008
Muccifora, S. (2008). Effects of copper on spore germination, growth and ultrastructure of Polypodium cambricum L. gametophytes. ENVIRONMENTAL POLLUTION, 153(2), 369-375 [10.1016/j.envpol.2007.08.012].
File in questo prodotto:
File Dimensione Formato  
copper.pdf

non disponibili

Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 1.64 MB
Formato Adobe PDF
1.64 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/24302