Contour-clamped homogeneous electrophoresis and an embedded-agarose method of sample preparation were combined to carry out an analysis of the chromosome sets of nine strains of Hansenula polymorpha (syn. Pichia angusta). Chromosomal DNA molecules could be separated into a series of bands ranging, approximately, from 650 up to 2,200 kb in size. Polymorphism of the electrophoretic pattern was demonstrated among the strains investigated in this study. Cross-hybridization between H. polymorpha and Saccharomyces cerevisiae ribosomal DNA was also observed.
Marri, L., Rossolini, G.M., Satta, G. (1993). Chromosome polymorphism among strains of Hansenula polymorpha (syn. Pichia angusta). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 59(3), 939-941.
Chromosome polymorphism among strains of Hansenula polymorpha (syn. Pichia angusta).
MARRI, LAURA;ROSSOLINI, GIAN MARIA;
1993-01-01
Abstract
Contour-clamped homogeneous electrophoresis and an embedded-agarose method of sample preparation were combined to carry out an analysis of the chromosome sets of nine strains of Hansenula polymorpha (syn. Pichia angusta). Chromosomal DNA molecules could be separated into a series of bands ranging, approximately, from 650 up to 2,200 kb in size. Polymorphism of the electrophoretic pattern was demonstrated among the strains investigated in this study. Cross-hybridization between H. polymorpha and Saccharomyces cerevisiae ribosomal DNA was also observed.
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https://hdl.handle.net/11365/24009
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