Sumoylation is a post-translational modification involved in the regulation of several cell functions. Recent studies suggest its involvement in spermatogenesis, but occurrence and function of SUMO (small ubiquitin-like modifier) in mature spermatozoa remain unknown. We report the occurrence of several SUMO1-conjugated proteins, in a range of 20-85kDa, in ejaculated spermatozoa. By cytofluorimetric analysis, we evaluated the percentage of SUMO1-positive spermatozoa in 58 subjects undergoing semen analysis in our laboratory and correlated the obtained values with semen parameters. We found that the percentage of SUMO1-positive spermatozoa was inversely correlated with total (r=-0.35, p<0.01) and progressive motility (r=-0.29, p<0.05). Such correlations become stricter when only asthenospermic subjects were included in the analysis (r=-0.58, p=0.01 for progressive motility, n=17) and were lost in non-asthenospermic subjects. By immunofluorescence and immunoconfocal fluorescence, we demonstrated that SUMO1 is mainly located in the nucleus and, occasionally, in the midpiece of spermatozoa. Immunoelectron microscopy as well as a long permeabilization protocol demonstrated a massive localization of SUMO-1 in the nucleus. By using a fluorescent probe to distinguish dead/live cells, we show that SUMO1 is mainly present in live spermatozoa. In conclusion, sumoylation of human spermatozoa may be involved in the regulation of motility. © 2010 The Authors. International Journal of Andrology © 2011 European Academy of Andrology.
Marchiani, S., Tamburrini, L., Giuliano, L., Nosi, D., Sarli, V., Gandini, L., et al. (2011). Sumo1-ylation of human spermatozoa and its relationship with semen quality. INTERNATIONAL JOURNAL OF ANDROLOGY, 34(6 part 1), 581-593 [10.1111/j. 1365-2605.2010.01118.x].
Sumo1-ylation of human spermatozoa and its relationship with semen quality
Piomboni, Paola;
2011-01-01
Abstract
Sumoylation is a post-translational modification involved in the regulation of several cell functions. Recent studies suggest its involvement in spermatogenesis, but occurrence and function of SUMO (small ubiquitin-like modifier) in mature spermatozoa remain unknown. We report the occurrence of several SUMO1-conjugated proteins, in a range of 20-85kDa, in ejaculated spermatozoa. By cytofluorimetric analysis, we evaluated the percentage of SUMO1-positive spermatozoa in 58 subjects undergoing semen analysis in our laboratory and correlated the obtained values with semen parameters. We found that the percentage of SUMO1-positive spermatozoa was inversely correlated with total (r=-0.35, p<0.01) and progressive motility (r=-0.29, p<0.05). Such correlations become stricter when only asthenospermic subjects were included in the analysis (r=-0.58, p=0.01 for progressive motility, n=17) and were lost in non-asthenospermic subjects. By immunofluorescence and immunoconfocal fluorescence, we demonstrated that SUMO1 is mainly located in the nucleus and, occasionally, in the midpiece of spermatozoa. Immunoelectron microscopy as well as a long permeabilization protocol demonstrated a massive localization of SUMO-1 in the nucleus. By using a fluorescent probe to distinguish dead/live cells, we show that SUMO1 is mainly present in live spermatozoa. In conclusion, sumoylation of human spermatozoa may be involved in the regulation of motility. © 2010 The Authors. International Journal of Andrology © 2011 European Academy of Andrology.File | Dimensione | Formato | |
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