This paper presents a novel approach to study in vivo cellular kinetics by the combined use of magnetic resonance (MR) spectroscopy and Odum’s system modeling, which includes the entire process of substrate degradation and product formation. The experiment was conducted directly in the MR-probe, where microorganisms (Klebsiella planticola) were incubated with high 13C-glucose and 13C-xylose concentration. Sugar consumptions and 13C-ethanol production were followed for 17 h and MR-spectra were acquired every 30 min without external sampling. Fitting of data allowed evaluation of sugar degradation, ethanol formation, cell activation induced by substrates, as well as cell inhibition induced by ethanol. The dynamics related to the diauxic growth of the bacterium under study were analyzed in terms of different kinetic behaviors. This approach gives not only a good fit of theoretical to experimental results, but is able also to interpret the way substrates, cells and products interact with each other. The proposed methodology can be applied to different living systems to study complex novel mechanisms.

Gastaldelli, A., Martini, S., Donati, A., Marchettini, N., Rossi, C., & Bastianoni, S. (2009). A new approach to study in vivo cellular metabolism using a modellistic analysis of magnetic resounance spectra. MATHEMATICAL BIOSCIENCES, 222(1), 36-41 [10.1016/j.mbs.2009.08.005].

A new approach to study in vivo cellular metabolism using a modellistic analysis of magnetic resounance spectra

MARTINI, SILVIA;DONATI, ALESSANDRO;MARCHETTINI, NADIA;ROSSI, CLAUDIO;BASTIANONI, SIMONE
2009

Abstract

This paper presents a novel approach to study in vivo cellular kinetics by the combined use of magnetic resonance (MR) spectroscopy and Odum’s system modeling, which includes the entire process of substrate degradation and product formation. The experiment was conducted directly in the MR-probe, where microorganisms (Klebsiella planticola) were incubated with high 13C-glucose and 13C-xylose concentration. Sugar consumptions and 13C-ethanol production were followed for 17 h and MR-spectra were acquired every 30 min without external sampling. Fitting of data allowed evaluation of sugar degradation, ethanol formation, cell activation induced by substrates, as well as cell inhibition induced by ethanol. The dynamics related to the diauxic growth of the bacterium under study were analyzed in terms of different kinetic behaviors. This approach gives not only a good fit of theoretical to experimental results, but is able also to interpret the way substrates, cells and products interact with each other. The proposed methodology can be applied to different living systems to study complex novel mechanisms.
File in questo prodotto:
File Dimensione Formato  
Gastaldelli_et_al_MBS_2009.pdf

non disponibili

Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 806.58 kB
Formato Adobe PDF
806.58 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/23511