Inflammation plays an important role in the onset of angiogenesis. In the present study, we show that osteopontin (OPN), a proinflammatory mediator involved in tissue repair, induces IL-1 beta up-regulation in human monocytes. This was accompanied by the enhanced production of TNF-alpha, IL-8, and IL-6 a decreased release of IL-10, and increased p38 phosphorylation. The supernatants of OPN-treated monocytes were highly angiogenic when delivered on the chick embryo chorioallantoic membrane. The angiogenic response was completely abrogated by a neutralizing anti-IL-1Ab thus indicating that thin cytokine represents the major proangiogenic factor expressed by OPN-activated monocytes. Accordingly, rIL-1 beta mimicked the proangiogenic activity of OPN-treated monocyte supernatants, and IL-1R (type I) was found to be expressed in the chorioallantoic membrane. In conclusion, OPN-activated monocytes may contribute to the onset of angiogenesis through a mechanism mediated by IL-1 beta.

Naldini, A., Leali, D., Pucci, A., Morena, E., Carraro, F., Nico, B., et al. (2006). Cutting edge: IL-1beta mediates the proangiogenic activity of osteopontin-activated human monocytes. JOURNAL OF IMMUNOLOGY, 177(7), 4267-4270 [10.4049/jimmunol.177.7.4267].

Cutting edge: IL-1beta mediates the proangiogenic activity of osteopontin-activated human monocytes

NALDINI, A.;PUCCI, A.;MORENA, E.;CARRARO, F.;
2006-01-01

Abstract

Inflammation plays an important role in the onset of angiogenesis. In the present study, we show that osteopontin (OPN), a proinflammatory mediator involved in tissue repair, induces IL-1 beta up-regulation in human monocytes. This was accompanied by the enhanced production of TNF-alpha, IL-8, and IL-6 a decreased release of IL-10, and increased p38 phosphorylation. The supernatants of OPN-treated monocytes were highly angiogenic when delivered on the chick embryo chorioallantoic membrane. The angiogenic response was completely abrogated by a neutralizing anti-IL-1Ab thus indicating that thin cytokine represents the major proangiogenic factor expressed by OPN-activated monocytes. Accordingly, rIL-1 beta mimicked the proangiogenic activity of OPN-treated monocyte supernatants, and IL-1R (type I) was found to be expressed in the chorioallantoic membrane. In conclusion, OPN-activated monocytes may contribute to the onset of angiogenesis through a mechanism mediated by IL-1 beta.
Naldini, A., Leali, D., Pucci, A., Morena, E., Carraro, F., Nico, B., et al. (2006). Cutting edge: IL-1beta mediates the proangiogenic activity of osteopontin-activated human monocytes. JOURNAL OF IMMUNOLOGY, 177(7), 4267-4270 [10.4049/jimmunol.177.7.4267].
File in questo prodotto:
File Dimensione Formato  
J Immunol-2006-Naldini.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 855.45 kB
Formato Adobe PDF
855.45 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/22533
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo