teroid hormones, cytokines, and growth factors have a major role in evoking local endometrial changes needed for trophoblast implantation. In the present study, the effect of interleukin-1β (IL-1β), 17-β estradiol (E2), and progesterone (Pr) on activin A and follistatin (FS) secretion from cultured human endometrial stromal cells (HESCs) is evaluated. HESCs were obtained from healthy human endometrial samples (n = 8) collected from healthy women. The cells were cultured and stimulated with E2 (10 -7 M, 10-6M), Pr (10-7M, 10-6M), IL-1β (500 pg/mL), IL-1β (500 pg/mL) + E2 (10-6M), and IL-1β (500 pg/mL) + Pr (10-6M). Activin A and FS secretion and mRNA expression were assayed by enzyme-linked immunosorbent assay and semiquantitative reverse transcriptase-polymerase chain reaction, respectively. Pr (10-7 M, 10-6 M) significantly increased activin A secretion and mRNA expression from HESCs, but E2 did not show remarkable effects. The addition of IL-1β (P<.001), IL- 1β + E2 (P <.01), and IL-1β + Pr (P<.001). significantly stimulated activin A secretion and mRNA expression, compared to untreated cells. Activin A expression and secretion after the coincubation of IL-1β+ Pr were significantly higher than after IL-1β and IL-1β+ E2 stimuli ( P<.01 and P<.001, respectively). Neither Pr nor E2 and IL-1β had a significant effect on FS secretion and expression. IL-1β and Pr stimulated activin A but not FS secretion from cultured HESCs, and the effect of IL-1β was augmented by Pr. These findings, together with the evidence that activin A is involved in trophoblast implantation, suggest the existence of a complex cross-talk by which the ovary, through Pr secretion, and the embryo, through IL-1β production, may trigger the endometrial induction of activin A and consequently timing implantation. © 2007 by the Society for Gynecologic Investigation.

Florio, P., Rossi, M., Vigano, P., Luisi, S., Torricelli, M., Torres, P.B., et al. (2007). Interleukin 1beta and progesterone stimulate activin a expression and secretion from cultured human endometrial stromal cells. REPRODUCTIVE SCIENCES, 14(1), 29-36 [10.1177/1933719106298191].

Interleukin 1beta and progesterone stimulate activin a expression and secretion from cultured human endometrial stromal cells

FLORIO, P.;LUISI, S.;TORRICELLI, M.;
2007-01-01

Abstract

teroid hormones, cytokines, and growth factors have a major role in evoking local endometrial changes needed for trophoblast implantation. In the present study, the effect of interleukin-1β (IL-1β), 17-β estradiol (E2), and progesterone (Pr) on activin A and follistatin (FS) secretion from cultured human endometrial stromal cells (HESCs) is evaluated. HESCs were obtained from healthy human endometrial samples (n = 8) collected from healthy women. The cells were cultured and stimulated with E2 (10 -7 M, 10-6M), Pr (10-7M, 10-6M), IL-1β (500 pg/mL), IL-1β (500 pg/mL) + E2 (10-6M), and IL-1β (500 pg/mL) + Pr (10-6M). Activin A and FS secretion and mRNA expression were assayed by enzyme-linked immunosorbent assay and semiquantitative reverse transcriptase-polymerase chain reaction, respectively. Pr (10-7 M, 10-6 M) significantly increased activin A secretion and mRNA expression from HESCs, but E2 did not show remarkable effects. The addition of IL-1β (P<.001), IL- 1β + E2 (P <.01), and IL-1β + Pr (P<.001). significantly stimulated activin A secretion and mRNA expression, compared to untreated cells. Activin A expression and secretion after the coincubation of IL-1β+ Pr were significantly higher than after IL-1β and IL-1β+ E2 stimuli ( P<.01 and P<.001, respectively). Neither Pr nor E2 and IL-1β had a significant effect on FS secretion and expression. IL-1β and Pr stimulated activin A but not FS secretion from cultured HESCs, and the effect of IL-1β was augmented by Pr. These findings, together with the evidence that activin A is involved in trophoblast implantation, suggest the existence of a complex cross-talk by which the ovary, through Pr secretion, and the embryo, through IL-1β production, may trigger the endometrial induction of activin A and consequently timing implantation. © 2007 by the Society for Gynecologic Investigation.
2007
Florio, P., Rossi, M., Vigano, P., Luisi, S., Torricelli, M., Torres, P.B., et al. (2007). Interleukin 1beta and progesterone stimulate activin a expression and secretion from cultured human endometrial stromal cells. REPRODUCTIVE SCIENCES, 14(1), 29-36 [10.1177/1933719106298191].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/22261
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