Mature B cells are grouped into two major subsets, B-1 and B-2, believed to derive from separate lineages. We have recently shown that B-1 cells, which are characterized by CD5 surface expression, specifically exhibit significant levels of the tyrosine kinase Lck in man. Here we show that also in mice Lck expression is restricted to B-1 cells and address the potential role of Lck in B-1 cell development and activation. Using as a model an Lck–/– mouse, we show that, while dispensable for B-1 cell development, Lck is required for full and sustained activation of the tyrosine phosphorylation and MAP kinase cascades triggered by the BCR in CD5+, B-1 cells. The data suggest a potential role for Lck in the achievement of the higher activation threshold required for productive BCR signaling in B-1 as compared to B-2 cells.

Ulivieri, C., Valensin, S., Majolini, M.B., Matthews, R.J., Baldari, C.T. (2003). Normal B-1 cell development but defective BCR signaling in Lck-/- mice. EUROPEAN JOURNAL OF IMMUNOLOGY, 33(2), 441-445 [10.1002/immu.200310019].

Normal B-1 cell development but defective BCR signaling in Lck-/- mice

Ulivieri, Cristina;Baldari, Cosima T.
2003-01-01

Abstract

Mature B cells are grouped into two major subsets, B-1 and B-2, believed to derive from separate lineages. We have recently shown that B-1 cells, which are characterized by CD5 surface expression, specifically exhibit significant levels of the tyrosine kinase Lck in man. Here we show that also in mice Lck expression is restricted to B-1 cells and address the potential role of Lck in B-1 cell development and activation. Using as a model an Lck–/– mouse, we show that, while dispensable for B-1 cell development, Lck is required for full and sustained activation of the tyrosine phosphorylation and MAP kinase cascades triggered by the BCR in CD5+, B-1 cells. The data suggest a potential role for Lck in the achievement of the higher activation threshold required for productive BCR signaling in B-1 as compared to B-2 cells.
2003
Ulivieri, C., Valensin, S., Majolini, M.B., Matthews, R.J., Baldari, C.T. (2003). Normal B-1 cell development but defective BCR signaling in Lck-/- mice. EUROPEAN JOURNAL OF IMMUNOLOGY, 33(2), 441-445 [10.1002/immu.200310019].
File in questo prodotto:
File Dimensione Formato  
Ulivieri et al Eur. J. immunol. 2003.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 169.75 kB
Formato Adobe PDF
169.75 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/21917
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo