Glutathione-S-transferase and glutathione in the liver and blood of rats poisoned by thioacetamide [Contenuto di glutatione-S-transferasi e di glutatione nel fegato e nel sangue di ratti intossicati con tioacetamide]

In rats treated with thioacetamide (250 mg/Kg i.p.) the glutathione-S-transferase activity in liver and in plasma and the glutathione content in liver have been determined at various times. The glutathione-S-transferase activity in liver supernatant decreases after 24 and 48 hours and returns to normal values after 4 days. The glutathione-S-transferase activity in liver supernatant is not inhibited by thioacetamide at 20 mM. The glutathione-S-transferase activity appears in plasma after 12 hours, with a maximum at 24 hours, followed to a decrease toward zero levels; this activity can be determined in plasma even at a dose of 10 mg/Kg of thioacetamide. The glutathione content in liver increases at 48 and 96 hours after drug administration.