The development of safer analogues of immunosuppressants such as cyclosporin A and FK506 is an important goal for a number of clinical applications ranging from transplantation to the treatment of autoimmune diseases. Here we show the generation and the characterization of Jurkat T cell lines stably transfected with a reporter construct containing the firefly luciferase gene under the control of NF-AT. These lines specifically respond in a cyclosporin A-sensitive manner to T cell antigen receptor-derived signals. Due to the high levels of luciferase activity expression fewer than 1000 cells are required for detection of luciferase. In addition, a simplified luciferase assay allows to reduce both the manipulations and the time required for the assay, making these lines potentially useful models for the automated screening of cyclosporin A and FK506 analogues.
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|Titolo:||NF-AT-luciferase reporter T-cell lines as tools to screen immunosuppressive drugs|
|Citazione:||Baldari, C., DI SOMMA, M.M., MAJOLINI M., B., Ulivieri, C., Milia, E., & Telford, J.L. (1998). NF-AT-luciferase reporter T-cell lines as tools to screen immunosuppressive drugs. BIOLOGICALS, 26(1), 1-5.|
|Appare nelle tipologie:||1.1 Articolo in rivista|