Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a templatefree self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.

Rodrigues Martins, A., Riparbelli, M.G., Callaini, G., Glover, D.M., Bettencourt, D. (2007). Revisiting the Role of the Mother Centriole in Centriole Biogenesis. SCIENCE, 316(5827), 1046-1050 [10.1126/science.1142950].

Revisiting the Role of the Mother Centriole in Centriole Biogenesis

Riparbelli, M. G.;Callaini, G.;
2007-01-01

Abstract

Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a templatefree self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.
2007
Rodrigues Martins, A., Riparbelli, M.G., Callaini, G., Glover, D.M., Bettencourt, D. (2007). Revisiting the Role of the Mother Centriole in Centriole Biogenesis. SCIENCE, 316(5827), 1046-1050 [10.1126/science.1142950].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/21308