To investigate the phosphorylation capability of serogroup A Neisseria meningitidis (MenA) and to implement our knowledge in meningococcal biology and in bacterial post-translational modifications, cell extracts were separated by 2-DE and 51 novel phosphoproteins were revealed by the use of the highly specific Ser/Thr/Tyr-phosphorylated proteins staining by Pro-Q Diamond and identified by MALDI-ToF/MS. Our results indicate that phosphorylation in MenA is comparable to that of other bacterial species. A first functional characterization of the identified modified proteins was also given, in order to understand their role in meningococcal physiopathology.

Bernardini, G., Laschi, M., Serchi, T., Arena, S., D'Ambrosio, C., Braconi, D., et al. (2011). Mapping phosphoproteins in Neisseria meningitidis serogroup A. PROTEOMICS, 11(7), 1351-1358 [10.1002/pmic.201000406].

Mapping phosphoproteins in Neisseria meningitidis serogroup A

Bernardini, Giulia;Laschi, Marcella;Braconi, Daniela;Santucci, Annalisa
2011-01-01

Abstract

To investigate the phosphorylation capability of serogroup A Neisseria meningitidis (MenA) and to implement our knowledge in meningococcal biology and in bacterial post-translational modifications, cell extracts were separated by 2-DE and 51 novel phosphoproteins were revealed by the use of the highly specific Ser/Thr/Tyr-phosphorylated proteins staining by Pro-Q Diamond and identified by MALDI-ToF/MS. Our results indicate that phosphorylation in MenA is comparable to that of other bacterial species. A first functional characterization of the identified modified proteins was also given, in order to understand their role in meningococcal physiopathology.
2011
Bernardini, G., Laschi, M., Serchi, T., Arena, S., D'Ambrosio, C., Braconi, D., et al. (2011). Mapping phosphoproteins in Neisseria meningitidis serogroup A. PROTEOMICS, 11(7), 1351-1358 [10.1002/pmic.201000406].
File in questo prodotto:
File Dimensione Formato  
Mapping phosphoproteins in Neisseria meningitidis serogroup A.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 122.9 kB
Formato Adobe PDF
122.9 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
pmic_201000406sm.pdf

non disponibili

Tipologia: Altro materiale allegato
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 692.93 kB
Formato Adobe PDF
692.93 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/21097
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo