Ca(2+) channel properties of the mink ryanodine receptor type 3 (RyR3), expressed in HEK293 cells, were studied in planar lipid bilayers to which RyR3 rich membrane fragments had been fused. RyR3 channels were not active at resting levels of Ca(2+)(free) but were gated by an additional 1 mM ATP, exhibiting long open times. The second major finding was the absence of channel inactivation at millimolar Ca(2+)(free). Insertion of a myc tag at the N-terminus of RyR3 did not affect the channel properties. As to skeletal muscle, the observed type 3 channel properties appear physiologically meaningful by assisting type 1 channels in calcium release.
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|Titolo:||ATP-induced activation of expressed RyR3 at low free calcium.|
|Citazione:||M., M., Rossi, D., I., S., E., B., C., R., Sorrentino, V., et al. (2000). ATP-induced activation of expressed RyR3 at low free calcium. FEBS LETTERS, 471, 256-260.|
|Appare nelle tipologie:||1.1 Articolo in rivista|