Effect of 5-100 mu M epigallocatechin gallate (EGCG) on hepatic glucose 6-phosphatase (G6Pase) system was investigated. EGCG inhibited G6Pase in intact but not in permeabilized rat liver microsomes, suggesting the interference with the transport. However, EGCG did not hinder microsomal glucose 6-phosphate (G6P) uptake. Instead, it increased the accumulation of radioactivity after the addition of [C-14]IG6P, presumably due to a slower release of [C-14]glucose, the product of luminal hydrolysis. Indeed, EGCG was found to inhibit microsomal glucose efflux. Since G6Pase activity is depressed by glucose in a concentration-dependent manner, we concluded that EGCG inhibits G6Pase through an elevated luminal glucose level.
|Titolo:||Inhibition of hepatic glucose 6-phosphatase system by the green tea flavanol epigallocatechin gallate|
|Citazione:||Csala, M., Margittai, E., Senesi, S., Gamberucci, A., Banhegyi, G., Mandl, J., et al. (2007). Inhibition of hepatic glucose 6-phosphatase system by the green tea flavanol epigallocatechin gallate. FEBS LETTERS, 581(8), 1693-1698.|
|Appare nelle tipologie:||1.1 Articolo in rivista|